两种种源八角花色差异性的转录组分析  

Transcriptome Analysis of the Formation of Flower Color in Star Anise(Illicium verum)

作　　者：曾祥艳[1] 李开祥[1] 黄开顺[1] 何小燕 杨卓颖 梁文汇[1] 赵志珩[1] Zeng Xiangyan;Li Kaixiang;Huang Kaishun;He Xiaoyan;Yang Zhuoying;Liang Wenhui;Zhao Zhiheng(Guangxi Key Laboratory of Special Non-wood Forest Cultivation&Utilization,Guangxi Engineering Technology Research Center of Woody Spices,National Forestry and Grassland Engineering Technology Research Center of Anise&Cinnamon,Guangxi Forestry Research Institute,Nanning,530002;Forestry Seed and Seedling Station of Yunnan Province,Kunming,650224)

机构地区：[1]广西壮族自治区林业科学研究院,国家林业和草原局八角肉桂工程技术研究中心,广西木本香料工程技术研究中心,广西特色经济林培育与利用重点实验室,南宁530002 [2]云南省林木种苗工作总站,昆明650224

出　　处：《分子植物育种》2024年第15期4965-4973,共9页Molecular Plant Breeding

基　　金：广西特色经济林培育与利用重点实验室自主课题(19-A-01-04);广西重点研发计划项目(桂科AB1850014;桂科AB18221040)共同资助。

摘　　要：花色是植物重要性状之一,目前尚未明确八角(Illicium verum)两种不同花色形成的原因。本研究以八角淡红花、红花两种种源为试验材料,利用RNA Sequencing (RNA-Seq)技术进行八角种源转录组基因表达分析,探索八角花色形成的分子调控网络基础。结果表明:八角淡红花与红花种源之间存在2 824个显著表达差异的基因,其中上调基因1 352个,下调基因1 472个。差异基因GO富集分析注释到3个大类共51个通路,KEGG富集分析注释到5个大类共130个通路,差异基因显著富集在次生代谢物的生物合成、花青素生物合成通路。本研究筛选与花青素生物合成相关的基因,发现共有8个差异基因与4个花青素合成途径中的结构基因4CL、CHI、DFR、3GT相结合且大部分表达上调,尤其3GT可能是决定八角花色加深的重要候选基因。本研究筛选到了八角淡红花与红花之间相关的差异基因,特别是花青素生物合成等方面的基因,揭示了花青素生物合成途径是八角花色加深的重要途径。Flower color is one of the important traits of plants. The reason for the formation of the two different color flowers of Illicium verum has not yet been clarified. To explore the metabolic pathways of flower color formation, RNA Sequencing(RNA-Seq) technology has been used to analyze the gene expression patterns of pink-colored flower and red-colored flower provenances. The results showed 2 824 genes with significant differences in expression between the provenances of pink-colored flower and red-colored flower were observed, among which1 352 were up-regulated genes and 1 472 were down-regulated genes. GO enrichment analysis showed that 51pathways were annotated into 3 categories, and KEGG enrichment analysis annotated 5 categories which were composed of 130 pathways. Differential genes were significantly enriched in the biosynthesis of secondary metabolites and anthocyanin biology. A total of 8 differential genes and structural genes 4CL, CHI, DFR, 3GT in the 4 anthocyanin synthesis pathways were screened, and most of them were up-regulated, especially 3GT. 3GT might be an important candidate gene that determines the darkening of the flower color in Illicium verum. In conclusion, we screened out the related differential genes between pink-colored flower and red-colored flower, especially genes related to anthocyanin biosynthesis, revealing that the anthocyanin biosynthetic pathway is an important way to deepen the color of the flower in Illicium verum.

关 键 词：八角(Illicium verum) 转录组 花青素合成 3GT 

分 类 号：S573[农业科学—作物学]