外源脱落酸对钩藤中生物碱合成的影响  

Effect of Exogenous Abscisic Acid on Biosynthesis of Alkaloids in Uncaria rhynchophylla

作　　者：周浩 强玮 敖雯雯 路星星 廖海民 李雪 王晓红 张明生 Zhou Hao;Qiang Wei;Ao Wenwen;Lu Xingxing;Liao Haimin;Li Xue;Wang Xiaohong;Zhang Mingsheng(Collaborative Innovation Center for Mountain Ecology and Agro-Bioengineering(CICMEAB),Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),College of Life Science,Institute of Agro-bioengineering,Guizhou University,Guiyang,550025)

机构地区：[1]贵州大学生命科学学院,农业生物工程研究院,山地植物资源保护与保护种质创新教育部重点实验室,山地生态与农业生物工程协同创新中心,贵阳550025

出　　处：《分子植物育种》2024年第15期5091-5097,共7页Molecular Plant Breeding

基　　金：贵州省生物学一流学科建设项目(GNYL[2017]009);国家重点研发计划课题(2016YFC0502604);贵州大学培育项目(贵大培育[2019]51号)、贵州大学引进人才科研项目(贵大人基合字[2017]58)共同资助;贵州省中药材现代产业技术体系建设项目(GZCYTX-02)。

摘　　要：为了探究外施ABA对钩藤中活性成分钩藤碱(RIN)和异钩藤碱(IRN)合成与积累以及生物碱合成途径基因表达的影响,以期进一步揭示RIN和IRN生物合成对ABA的响应机制。本研究以6月龄的钩藤为材料,外施不同浓度的ABA,利用HPLC测定不同浓度脱落酸处理后钩藤叶中RIN和IRN的含量积累情况,采用荧光定量PCR (RT-qPCR)分析合成途径中关键酶基因STR、TDC、SGD和G8H等基因的表达。结果表明,100μmol/L的ABA溶液最适于促进钩藤叶中生物碱的合成积累,处理后2 d两种生物碱含量就比对照提高了1倍;同时,q PCR显示合成途径中SGD、TDC、G8H和STR家族酶基因均有不同程度的上调。因此,合理外施ABA溶液有助于促进钩藤活性成分的合成与积累,提高钩藤的经济效益,对钩藤药材的高效优质种植管理提供了理论依据,也为阐明钩藤生物碱生物合成的分子调控机制提供了基础。The objective of this study was to investigate the effects of external application of ABA on the synthesis and accumulation of the active ingredients: rhynchophylline(RIN) and isorhynchophylline(IRN), as well as the gene expression of alkaloid synthesis pathways, in order to further reveal the biosynthesis of RIN and IRN in response to ABA mechanism. In this study, 6-month-old Uncaria rhynchophylla was applied with different concentrations of ABA, and the accumulation of RIN and IRN in U. rhynchophylla leaves after treatment with different concentrations of ABA was measured by HPLC, and analyze some key enzyme expression by fluorescence quantitative PCR(RT-qPCR). The expression of key enzyme genes STR, TDC, SGD and G8H in the synthetic pathway. The results showed that 100 μmol/L ABA solution was most suitable for promoting the synthesis and accumulation of alkaloids in U. rhynchophylla leaves. The content of 2 alkaloids increased by 1-fold compared with the control after 2 days treatment. At the same time, qPCR showed that SGD, TDC, G8H and STR family enzyme genes were all up-regulated to varying degrees. Therefore, reasonable external application of ABA solution will help promote the synthesis and accumulation of the ac tive ingredients of U. rhynchophylla, improve the economic benefits of U.rhynchophylla, provide a theoretical basis for the efficient and high-quality planting management of U. rhynchophylla, and also clarify the biosynthesis of U. rhynchophylla alkaloids laid the foundation for the molecular regulation mechanism.

关 键 词：钩藤 基因表达 萜类吲哚生物碱 脱落酸 

分 类 号：S567.19[农业科学—中草药栽培]