电针对动脉粥样硬化家兔腹腔巨噬细胞胆固醇逆转运受体表达的影响  

Effects of electroacupuncture on the expression of cholesterol reverse transport receptors in peritoneal mcrophages of atherosclerotic rabbits

作　　者：栾海燕 佟晓哲 张邵宁 赵莹莹 姜凤伟 李慧 韩婧姝 成泽东[2] 李菁媛 LUAN Haiyan;TONG Xiaozhe;ZHANG Shaoning;ZHAO Yingying;JIANG Fengwei;LI Hui;HAN Jingshu;CHENG Zedong;LI Jingyuan(The First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121001,China;Liaoning University of Traditional Chinese Medicine)

机构地区：[1]锦州医科大学附属第一医院,锦州121001 [2]辽宁中医药大学

出　　处：《北京中医药大学学报》2024年第7期1005-1011,共7页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.81674078);辽宁省博士科研启动基金项目(No.2021-BS-268)。

摘　　要：目的 观察电针对动脉粥样硬化(AS)家兔腹腔巨噬细胞胆固醇逆转运受体三磷酸腺苷结合盒转运蛋白A1(ABCA1)、三磷酸腺苷结合盒转运蛋白G1(ABCG1)、B类Ⅰ型清道夫受体(SR-BⅠ)mRNA及蛋白表达的影响,探究电针治疗AS的作用机制。方法 将26只雄性新西兰家兔按照随机数字表法分为阴性对照组(n=7)、造模组(n=19)。阴性对照组家兔给予普通饲料喂养,造模组采用高脂饲料与颈总动脉球囊损伤术相结合的方法制备AS模型。将造模成功的家兔按随机数字表法分为模型组、电针组和阿托伐他汀组,每组各6只。电针组家兔予“内关”“足三里”“关元”进行电针治疗,疏密波(4 Hz/20 Hz),电流为1 mA,每次留针20 min,1次/d,6 d为1个疗程,共4个疗程;阿托伐他汀组家兔灌胃阿托伐他汀钙片悬浊液(1 mg/kg),1次/d,6 d为1个疗程,共4个疗程。干预结束后,采用HE染色法观察家兔颈总动脉组织形态学变化;取家兔腹腔巨噬细胞,实时荧光PCR法检测ABCA1、 ABCG1、SR-BⅠ的mRNA表达水平;蛋白质印迹法检测ABCA1、 ABCG1、SR-BⅠ的蛋白表达水平。结果 阴性对照组家兔颈总动脉内膜平滑;模型组家兔颈总动脉内膜受损,管壁增厚,粥样斑块形成;电针组、阿托伐他汀组家兔管壁增厚情况改善明显,粥样斑块面积缩小。与阴性对照组比较,模型组家兔腹腔巨噬细胞中ABCA1、 ABCG1、SR-BⅠmRNA和蛋白表达均降低(均P<0.01);与模型组比较,电针组和阿托伐他汀组家兔腹腔巨噬细胞中ABCA1、 ABCG1、SR-BⅠmRNA和蛋白表达均升高(均P<0.01);与电针组比较,阿托伐他汀组家兔腹腔巨噬细胞中ABCG1、SR-BⅠmRNA升高,ABCA1、ABCG1、SR-BⅠ蛋白表达升高(均P<0.01)。结论 电针可上调AS家兔腹腔巨噬细胞ABCA1、 ABCG1、SR-BⅠmRNA及蛋白的表达,增强胆固醇逆转运过程,这可能是针刺治疗AS的机制。The objective of this study was to examine the effects of electroacupuncture on the expression of ATP-binding cassette transporter A1(ABCA1),ATP-binding cassette transporter G1(ABCG1),and class B type Ⅰ scavenger receptor(SR-BⅠ) genes and proteins in peritoneal macrophages of atherosclerotic rabbits.The study aimed to explore the mechanism underlying the treatment of atherosclerosis(AS) with electroacupuncture.Methods Twenty-six male New Zealand rabbits were randomly divided into the negative control group(n=7) and the modeling group(n=19) using a random number table method.The negative control group rabbits were fed a regular diet,while the modeling group was induced with a combination of high-fat feed and common carotid artery balloon injury surgery to create an AS model.After successful modeling,the rabbits in the modeling group were further divided into the model group,the electroacupuncture group,and the atorvastatin group,with 6 rabbits in each group.The rabbits in the electroacupuncture group received electroacupuncture at "'Neiguan'(PC6) ","'Zusanli'(ST36)",and "'Guanyuan'(ST25)" acupoints,using a density wave,a current of 1 mA,and a frequency of 4 Hz/20 Hz,once a day.The needle was retained for 20 minutes each time,and a total of 4 courses of treatment were conducted,with 6 days per course.The rabbits in the atorvastatin group were administered atorvastatin calcium tablet suspension(1 mg/kg) orally once a day,for 6 days per course,with a total of 4 courses.After the interventions,HE staining was performed to observe the morphological changes in the common carotid artery tissue of the rabbits.Peritoneal macrophages were collected from the rabbits,and the mRNA expression levels of ABCA1,ABCG1,and SR-BⅠ were measured using real-time fluorescence PCR.The protein expression levels of ABCA1,ABCG1,and SR-BⅠ were detected using Western blotting.Results The negative control group exhibited smooth intima of common carotid artery in rabbits,while the model group displayed damaged intima of common carotid ar

关 键 词：动脉粥样硬化 电针 胆固醇逆转运受体 家兔 

分 类 号：R246.1[医药卫生—针灸推拿学]