大柴胡汤含药血清通过JAK2/STAT3信号通路抑制AR42J细胞炎症反应  

Dachaihu Decoction medicated serum inhibiting the AR42J cells inflflammatory response by inhibiting the JAK2/STAT3 signaling pathway

作　　者：赵东颖 王清华[2] 于艳[2] 姚旭[2] ZHAO Dongying;WANG Qinghua;YU Yan;YAO Xu(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China)

机构地区：[1]辽宁中医药大学,沈阳110847 [2]辽宁中医药大学附属医院,沈阳110032

出　　处：《吉林中医药》2024年第8期960-964,共5页Jilin Journal of Chinese Medicine

基　　金：辽宁省自然科学基金项目(2019-MS-222)。

摘　　要：目的探讨大柴胡汤含药血清对胰腺腺泡细胞炎症反应的防治作用及可能机制。方法HPLC法检测大柴胡汤中指标成分含量;采用雨蛙肽处理AR42J细胞复制急性腺泡细胞炎症模型,采用CCK-8方法检测大柴胡汤含药血清对细胞活力的影响;Western blot方法检测细胞内JAK2、STAT3及NFκB磷酸化程度;ELISA法检测IL-6和TNF-α含量。结果大柴胡汤中黄芩苷和芍药苷含量分别为13.02 mg/mL、7.19 mg/mL,大柴胡汤含药血清用量不超过20%;大柴胡汤含药血清降低炎性细胞上清液中TNF-α和IL-6含量,降低细胞内JAK2、STAT3及NFκB磷酸化作用,减轻细胞炎症反应;STAT3抑制剂cucurbitacin可减弱大柴胡汤的炎症抑制作用。结论大柴胡汤含药血清可减轻腺泡细胞急性炎症作用,其机制可能为抑制细胞内JAK2/STAT3信号通路进而降低NFκB活性,减少促炎性细胞因子合成。Objective To investigate the preventive and therapeutic effects of Dachaihu Decoction(DCH)medicated serum on the inflammatory reaction of pancreatic acinar cells and its possible mechanisms.Methods HPLC was used to detect the contents of index components in Dasaihu Decoction.The acute acinar cell inflammation model was replicated by inducing the AR42J cell with cearulein.Cell viability of different concentrations of DCH-medicated serum was detected by CCK-8.The phosphorylation levels of JAK2,STAT3 and NFκB were detected by Western blot.The contents of IL-6 and TNF-αwere detected by ELISA.Results The contents of baicalin and paeoniflorin in Dachaihu Decoction were 13.02 mg/mL and 7.19 mg/mL,respectively.The dosage of DCH-medicated serum was less than 20%.The DCH-medicated serum decreased the contents of TNF-αand IL-6 in the supernatant of inflammatory cells,relieved the phosphorylation of JAK2,STAT3 and NFκB in cells and alleviated the inflammatory response of cells.STAT3 inhibitor cucurbitacin decreased the inflammatory inhibitory effect of Dachaihu Decoction.Conclusion Dachaihu Decoction medicated serum can reduce the acute inflammation in acinous cells by inhibiting the JAK2/STAT3 signaling pathway and further reducing the activity of NFκB,and decreasing the synthesis of pro-inflammatory cytokines.

关 键 词：大柴胡汤 急性胰腺炎 酪氨酸蛋白激酶2 信号传导与转录激活因子3 白介素-6 核因子ΚB 

分 类 号：R285[医药卫生—中药学]