高效液相色谱法对比观察不同中药对发芽糙米中γ-氨基丁酸含量的影响  

Comparative study on the effect of different Chinese herbs onγ-aminobutyric acid contents in germinated brown rice using HPLC method

作　　者：詹杰[1] 刘乃榕 李景辉[1] 魏瑞娇 ZHAN Jie;LIU Nairong;LI Jinghui;WEI Ruijiao(Institute of Basic Medicine of Liaoning Province,Shenyang 110101,China;Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China)

机构地区：[1]辽宁省基础医学研究所,沈阳110101 [2]辽宁中医药大学,沈阳110032

出　　处：《中国中西医结合儿科学》2024年第4期291-296,共6页Chinese Pediatrics of Integrated Traditional and Western Medicine

基　　金：辽宁省教育厅基本科研项目(LJKMZ20222204)。

摘　　要：目的依据体质分析理论,对比分析不同药食两用类中药对发芽糙米中γ-氨基丁酸含量的影响,以期为不同体质儿童研发糙米药膳食品提供理论依据。方法实验分空白组(糙米组)、对照组(发芽糙米组)及中药发芽糙米组。中药发芽糙米组又分为9个亚组,分别为补气类(黄芪米、山药米、白扁豆米),滋阴类(玉竹米、黄精米、枸杞米、桑叶米),补血类(桑椹米、龙眼肉米),助阳类(杜仲米、干姜米),理气类(陈皮米、甘松米),化痰类(杏仁米、紫苏子米、莱菔子米),活血类(桃仁米、姜黄米),利水渗湿类(茯苓米、薏苡仁米),清利类(决明子米、夏枯草米、荷叶米、淡豆豉米)。其中补气类、助阳类、滋阴类和补血类为补益类中药发芽糙米,利水渗湿类、化痰类、清热类、理气类和活血类为泻实类中药发芽糙米。采用高效液相色谱法(HPLC)定量分析糙米、发芽糙米和不同复方中药发芽糙米和中药中γ-氨基丁酸(GABA)含量,建立γ-氨基丁酸质量浓度与吸光度值的线性回归方程,计算并对比分析样品中γ-氨基丁酸含量。结果与空白组比较,除杜仲米、甘松米和桃仁米外的其他各组发芽糙米中γ-氨基丁酸含量均明显高于空白组(P<0.05)。与对照组比较,黄芪、山药、白扁豆、玉竹、黄精、枸杞、桑叶、桑椹、龙眼肉、薏苡仁、杏仁、紫苏子、莱菔子、淡豆豉、陈皮、姜黄发芽糙米中γ-氨基丁酸含量明显提高(P<0.05)。干姜、茯苓、决明子、夏枯草、荷叶发芽糙米中γ-氨基丁酸含量高于糙米,但低于普通发芽糙米,差异有统计学意义(P<0.05)。按中药不同功能类别比较,补益组中补气类、滋阴类、补血类中药发芽糙米中γ-氨基丁酸含量均明显高于助阳类(P<0.05);泻实组中理气类和化痰类中药发芽糙米中γ-氨基丁酸含量明显高于活血类(P<0.05)。黄芪、黄精、桑椹、龙眼肉、陈皮、桑叶中γ-氨基Objective Based on the theory of constitution analysis,to compare and analyze the effects of different kinds of medicinal and edible herbs on the content of GABA in germinated brown rice,so as to provide theoretical basis for the development of medicinal diet of brown rice for children with different constitutions.Methods The experiment included three groups:blank group(brown rice group),control group(germinated brown rice group)and TCM germinated brown rice group.The TCM germinated brown rice group was again divided into 9 subgroups:qi-tonifying group(Huang Qi rice,Shan Yao rice,Bai Bian Dou rice),yin-nourishing group(Yu Zhu rice,Huang Jing rice,Gou Qi rice,Sang Ye rice),blood-supplementing group(Sang Shen rice,Long Yan Rou rice),yang-assisting group(Du Zhong rice,Gan Jiang rice),qi-regulating group(Chen Pi rice,Gan Song rice),sputum-resolving group(Xing Ren rice,Zi Su Zi rice,Lai Fu Zi rice),blood-activating group(Tao Ren rice,Jiang Huang rice),dampness-eliminating group(Fu Ling rice,Yi Yi Ren rice),and heat-clearing group(Jue Ming Zi rice,Xia Ku Cao rice,He Ye rice,Dan Dou Chi rice).Among them,the qi-tonifying group,yang-assisting group,yin-nourishing group and blood-supplementing group involved germinated brown rice with Chinese herbs for invigoration,and dampness-eliminating group,sputum-resolving group,heat-clearing group,qi-regulating group and blood-activating group involved germinated brown rice with Chinese herbs for reducing excess.HPLC method was used to quantitatively analyze the contents of GABA in brown rice,germinated brown rice and germinated brown rice with different compound of Chinese herbs,a linear regression equation of the mass concentration of GABA and the absorbance value was established,and the GABA content in the samples was calculated and compared.Results Compared with blank group,the content of GABA was significantly higher in all groups of germinated brown rice except for DU Zhong rice,Gan Song rice and Tao Ren rice(P<0.05).Compared with control group,the content of GABA significantl

关 键 词：发芽糙米 中药 Γ-氨基丁酸 高效液相色谱 含量测定 

分 类 号：R285.5[医药卫生—中药学]