基于“敲除策略”筛选高良姜乙酸乙酯部位抗幽门螺杆菌胃炎活性成分  

Screening of Anti-Helicobacter pylori Gastritis Active Component of the Ethyl Acetate Extract of Alpinia officinarum Hance Based on the Knockout Strategy

作　　者：罗鑫 郑吴殷晓 詹健婷 杨敬宇 马浩然 干国平 尤朋涛 叶晓川 刘丹 LUO Xin;ZHENG Wuyingxiao;ZHAN Jianting;YANG Jingyu;MA Haoran;GAN Guoping;YOU Pengtao;YE Xiaochuan;LIU Dan(Hubei Key Laboratory of Resource and Chemistry of Chinese Medicine,College of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065,China;Hubei Engineering Research Center for Traditional Chinese Medicine Processing,Wuhan 430065,China)

机构地区：[1]湖北中医药大学药学院中药资源与中药化学湖北省重点实验室,武汉430065 [2]湖北省中药炮制工程技术研究中心,武汉430065

出　　处：《医药导报》2024年第9期1387-1392,共6页Herald of Medicine

基　　金：国家自然科学基金资助项目(82173960、31400302)。

摘　　要：目的筛选高良姜乙酸乙酯部位抗幽门螺杆菌胃炎活性成分。方法联合“敲除策略”与高效液相色谱(HPLC)检测对高良姜乙酸乙酯部位的组分(成分)进行分离,同时得到不含该组分(成分)的阴性样品;构建幽门螺杆菌感染人胃黏膜上皮细胞(GES-1)胃炎模型,采用酶联免疫吸附测定(ELISA)法检测细胞上清液中白细胞介素(IL)-6、肿瘤坏死因子-α(TNF-α)、IL-8和IL-1β水平。结果总黄酮组分、不含二苯基庚烷的阴性组分、不含5-羟基-7-(4-羟基-3-甲氧基)苯基-1-苯基-3-庚酮(DHPA)的阴性组分以及高良姜素(给药浓度8μg·mL^(-1)作用24 h),均能够显著降低幽门螺杆菌胃炎细胞上清液中IL-6水平;总黄酮组分浓度为16μg·mL^(-1)时可显著抑制幽门螺杆菌感染GES-1胃炎细胞IL-6、TNF-α、IL-8和IL-1β的释放。结论总黄酮组分是高良姜乙酸乙酯部位抗幽门螺杆菌胃炎的主要活性组分,该研究结果为进一步阐释高良姜抗幽门螺杆菌胃炎药效物质基础奠定了基础。Objective To screen the anti-Helicobacter pylori gastritis active components of the ethyl acetate extract of Alpinia officinarum Hance.Methods The“knock-out”strategy combined with high-performance liquid chromatography(HPLC)detection was developed to separate the components of the ethyl acetate extract of A.officinarum while obtaining the negative samples without the components.A human gastric epithelial cell(GES-1)model of H.pylori gastritis was established,and the levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),interleukin-8(IL-8)and interleukin-1β(IL-1β)in the supernatant of the cells were determined by enzyme-linked immunosorbent assay(ELISA).Results The total flavonoid fraction,the negative fraction without total diphenylheptanoids,the negative fraction without 5-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-phenyl-3-heptanone(DHPA),and galangin significantly reduced IL-6 levels in the supernatant of H.pylori infected GES-1 cells at a concentration of 8μg·mL^(-1) with 24 h incubation.The total flavonoid fraction strongly inhibited the release of IL-6,TNF-α,IL-8,and IL-1βfrom H.pylori gastritis GES-1 cells at a concentration of 16μg·mL^(-1).Conclusions The total flavonoid fraction is the major anti-H.pylori gastritis active component of the ethyl acetate extract of A.officinarum.The results lay the foundation for further elucidation of the material basis of A.officnarum against H.pylori gastritis.

关 键 词：高良姜 幽门螺杆菌胃炎 敲除 活性组分 活性成分 

分 类 号：R282.71[医药卫生—中药学] R285.5[医药卫生—中医学]