DRVVT法检测狼疮抗凝物标准化流程的建立及应用结果分析  

Establishment and application of standard operation procedure for the entire process of lupus anticoagulant DRVVT detection

作　　者：但刚 孙巍 陈清红 胡莉娜 郭郑斌 张建华 张睿 刘媛 DAN Gang;SUN Wei;CHEN Qinghong;HU Lina;GUO Zhengbin;ZHANG Jianhua;ZHANG Rui;LIU Yuan(Department of Laboratory Medicine,the General Hospital of Western Theater Command PLA,Chengdu,Sichuan 610083,China)

机构地区：[1]中国人民解放军西部战区总医院检验科,四川成都610083

出　　处：《中华全科医学》2024年第8期1390-1394,共5页Chinese Journal of General Practice

基　　金：四川省科技厅重点研发项目(2021YJ0191)。

摘　　要：目的探讨建立使用稀释鲁赛尔蝰蛇毒时间(DRVVT)法检测狼疮抗凝物(LA)全流程的标准化程序,以保证LA检测结果准确性并为临床诊断治疗提供支撑。方法本研究遵循相关行业要求,包括《医疗机构实验室管理办法》和《临床血液学检验常规项目分析质量要求WS/T 406-2012》等,从DRVVT法检测LA整个流程包含检验前、检验中、检验后等阶段,对检测步骤进行全程标准化要求,建立以标准操作流程为核心的“DRVVT法狼疮抗凝物检测手册”,并对检测结果进行全流程的跟踪监控,以确保LA检测结果的准确性,并为临床诊断治疗提供支持。本研究对象为西部战区总医院2021—2023年进行LA检测的共计3595例标本,通过对送检标本量、送检科室分布、年度增长量等方面进行分析,探讨标准化检测流程建立后临床对此项目的认可程度、对疾病诊断准确度等方面的提升效果。结果通过建立一系列标准化的操作和检测流程,实现对检测结果有效监控,能准确反映患者体内LA的含量,为诊断和治疗提供有效支持。随着标准化流程的持续展开,临床对相关项目的认可度持续提升,送检科室和送检量持续上升。送检量从2021年的841例上升到2023年的1460例,增大幅度为73.0%;从送检科室的分布来看:2021年送检科室16个,2023年达到29个,增长幅度为81.3%;完全达到了支撑临床、与临床积极互动的良好效果。结论建立针对DRVVT法LA检测的标准化程序,可以为临床提供准确、真实反映病情的检测结果,加深与临床的沟通,并对相关性疾病的诊断和治疗起到积极的支持作用。Objective To investigate the standardized procedure for the entire process of lupus anticoagulant(LA)detection using the dilute Russell viper venom time(DRVVT)method to ensure the accuracy of LA test results and to aid in clinical diagnosis and treatment.Methods This study followed relevant industry requirements,including"Management Measures for Medical Institution Laboratory Management"and"Quality Requirements for Routine Analysis of Clinical Hematology Tests WS/T 406-2012".The entire process of LA detection using the DRVVT method,including pretest,test and posttest phases stages,was standardized.A"DRVVT Method Lupus Anticoagulant Detection Manual"was created based on standard operating procedures(SOP),and test results were tracked and monitored throughout the process to ensure the accuracy of LA test results and to aid in clinical diagnosis and treatment.The study included a total of 3595 specimens for LA testing in General Hospital of Western Theater Command PLA,from 2021 to 2023.By analyzing aspects such as the number of specimens submitted,the distribution of submitting departments and annual growth,the study examined the degree of clinical recognition of this project and the improvement in the accuracy of disease diagnosis after establishing a standardized testing process.Results By establishing a set of standardized operation and detection procedures,we can effectively monitor the test results,and accurately reflect the concentration of LA in patients,to provide effective support for diagnosis and treatment.With the continuous implementation of standardized procedures,the clinical recognition of relevant items has been improved,leading to a sustained increase in the number of specimens and departments submitting specimen.The number of specimens increased from 841 in 2021 to 1460 in 2023,with a growth rate of 73.0%.In terms of departments submitting specimen,there were 16 departments in 2021 and this number reached 29 departments in 2023,with a growth rate of 81.3%.This has fully achieved the desired effect

关 键 词：狼疮抗凝物 稀释鲁赛尔蝰蛇毒时间 标准化操作 

分 类 号：R593[医药卫生—内科学] R446.11[医药卫生—临床医学]