支气管哮喘并发肺部感染患儿血清HDAC1、KLF5水平及意义  

Levels and significance of serum HDAC1 and KLF5 in children with bronchial asthma complicated by pulmonary infection

作　　者：刘明华 徐金勇 王培培 LIU Minghua;XU Jinyong;WANG Peipei(Department of Pediatrics,Qingdao Municipal Hospital,Qingdao,Shandong 266002,China;Department of Emergency Pediatrics,Zhucheng People′s Hospital,Zhucheng,Shandong 262200,China)

机构地区：[1]山东省青岛市市立医院儿科,山东青岛266002 [2]山东省诸城市人民医院急诊儿科,山东诸城262200

出　　处：《检验医学与临床》2024年第16期2340-2344,共5页Laboratory Medicine and Clinic

基　　金：山东省医药卫生科技发展计划项目(202004070709)。

摘　　要：目的 探讨组蛋白去乙酰化酶1(HDAC1)、Kruppel样因子5(KLF5)在支气管哮喘(BA)并发肺部感染患儿血清中的水平及意义。方法 收集山东省青岛市市立医院2022年6月至2023年1月收治的140例BA患儿作为研究对象,根据是否并发肺部感染分为未感染组(79例)和感染组(61例);另选取同期在山东省青岛市市立医院体检健康儿童140例作为对照组。比较各组血清HDAC1、KLF5水平;采用多因素Logistic回归分析BA患儿并发肺部感染的影响因素;绘制受试者工作特征(ROC)曲线分析血清HDAC1、KLF5水平对BA患儿并发肺部感染的预测价值。结果 感染组血清HDAC1、KLF5水平显著高于未感染组和对照组(P<0.05);未感染组血清HDAC1、KLF5水平显著高于对照组(P<0.05)。感染组有哮喘家族史患者比例高于未感染组,病程长于未感染组,差异均有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,血清HDAC1、KLF5水平及哮喘家族史、病程均是BA患儿并发肺部感染的影响因素(P<0.05)。ROC曲线结果显示,HDAC1、KLF5二者联合预测BA患儿并发肺部感染的曲线下面积(AUC)为0.930,优于HDAC1、KLF5单独预测的AUC(Z=3.408,P=0.001;Z=2.539,P=0.011)。结论 BA并发肺部感染患儿血清HDAC1、KLF5水平显著升高,且HDAC1、KLF5联合检测对BA患儿并发肺部感染有较好的预测价值。Objective To investigate the levels and predictive values of serum histone deacetylase 1(HDAC1)and Kruppel like factor 5(KLF5)of children with bronchial asthma(BA)complicated with pulmonary infection.Methods A total of 140 children with BA who underwent treatment in Qingdao Municipal Hospital from June 2022 to January 2023 were collected as research subjects and divided into uninfected group(n=79)and infected group(n=61)based on whether they were complicated with pulmonary infection or not;while another 140 healthy individuals who underwent physical examination during the same period in Qingdao Municipal Hospital were selected as control group.The levels of serum HDAC1 and KLF5 in each group were compared;Multivariate Logistic regression analysis was applied to analyze the influencing factors of pulmonary infection in children with BA;Receiver operating characteristic(ROC)curve was applied to analyze the predictive value of serum HDAC1 and KLF5 levels for pulmonary infection in BA children.Results The serum levels of HDAC1 and KLF5 in the infected group were obviously higher than those in the uninfected group and the control group(P<0.05);the serum levels of HDAC1 and KLF5 in the uninfected group were obviously higher than those in the control group(P<0.05).The proportion of children with family history of asthma in the infected group was higher than that in the uninfected group,and the duration of the disease was longer than that in the uninfected group,with statistically significant differences(P<0.05).Multivariate Logistic regression analysis results showed that serum HDAC1,KLF5 levels,family history of asthma,and course of disease were the influencing factors for pulmonary infection in BA children(P<0.05).ROC curve results showed that the area under the curve of HDAC1 combined with KLF5 for pulmonary infection in BA children was 0.930,which was better than that of single detection of serum HDAC1 and KLF5(Z=3.408,P=0.001;Z=2.539,P=0.011).Conclusion The levels of serum HDAC1 and KLF5 in children with pulmonary

关 键 词：组蛋白去乙酰化酶1 Kruppel样因子5 支气管哮喘 肺部感染 儿童 

分 类 号：R725.6[医药卫生—儿科]