Proliferating cell nuclear antigen of Ulva prolifera is involved in the response to temperature stress  

作　　者：Hongyan HE Juanjuan YANG Yuan HE Xiuwen YANG Caiwei FU Dongren ZHANG Jingwei DONG Aurang ZEB Jing QU Songdong SHEN 

机构地区：[1]School of Biology&Basic Medical Sciences,Soochow University,Suzhou 215101,China [2]Department of Pathology,Affiliated Hospital and Medical School of Nantong University,Nantong 226001,China

出　　处：《Journal of Oceanology and Limnology》2024年第4期1227-1241,共15页海洋湖沼学报（英文）

基　　金：Supported by the National Natural Science Foundation of China(Nos.41976109,42276100);the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。

摘　　要：Ulva prolifera is the most common specie causative to green tide,and its growth is sensitive to temperature stress.However,the mechanisms of U.prolifera response to temperature stress remain elusive.In this study,high temperature(36℃)stimulus promoted the death of unformed cell wall protoplasts and delayed the division of formed cell wall protoplasts,while low-temperature(4℃)stimulus did not,suggesting that the mechanisms of the response of U.prolifera to high and low temperature stresses are different.Transcriptome results show that proliferation-related genes were differentially expressed under high and low-temperature stresses,especially the proliferating cell nuclear antigen(PCNA)and cyclins(CYCs).Subsequently,the interaction between PCNA and Cyclin A was confirmed by Co-immunoprecipitation,yeast two-hybrid,and so on.Furthermore,high-and low temperature stresses induced the expression of PCNA and Cyclin A in varying of degrees,and activated extracellular signal-regulated kinase(ERK)signal pathway.These results suggest,PCNA,Cyclin A,and ERK signal pathway played important roles in the resistance of U.prolifera to temperature stress.Interestingly,high-temperature stress induced an increase of miR-2916 in abundance,and exhibiting reverse expression of PCNA;and PCNA was target gene of miR-2916,suggesting that miR-2916 protected U.prolifera from high-temperature stress via post-transcriptionally regulation of PCNA.This study laid a foundation for understanding the function of PCNA and Cyclin A,moreover,it has a guiding significance to explore the mechanisms of the response to temperature stress from proliferation-related genes regulatory networks in U.prolifera.

关 键 词：UIva prolifera proliferating cell nuclear antigen Cyclin A extracellular signal-regulated kinase MICRORNA temperature stress 

分 类 号：S917.3[农业科学—水产科学]