Spatial compartmentalization and temporal stability of associated microbiota in Pacific oyster Crassostrea gigas  

作　　者：Qiang FU Zichao YU Junyan ZHAO Lei GAO Ning KONG Lingling WANG Linsheng SONG 

机构地区：[1]Liaoning Key Laboratory of Marine Animal Immunology and Disease Control,Dalian Ocean University,Dalian 116023,China [2]Functional Laboratory of Marine Fisheries Science and Food Production Processes,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China [3]Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai),Zhuhai 519000,China [4]Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control,Dalian Ocean University,Dalian 116023,China

出　　处：《Journal of Oceanology and Limnology》2024年第4期1348-1358,共11页海洋湖沼学报（英文）

基　　金：Supported by the National Natural Science Foundation of China(No.41961124009);the Earmarked Fund for China Agriculture Research System(No.CARS-49);the fund for Outstanding Talents and Innovative Team of Agricultural Scientific Research from MARA,the Innovation Team of Aquaculture Environment Safety from Liaoning Province(No.LT202009);the Dalian High Level Talent Innovation Support Program(No.2022RG14)。

摘　　要：The Pacific oyster Crassostrea gigas,one of the most exploited molluscs in the world,has suffered from massive mortality in recent decades,and the occurrence mechanisms have not been well characterized.In this study,to reveal the relationship of associated microbiota to the fitness of oysters,temporal dynamics of microbiota in the gill,hemolymph,and hepatopancreas of C.gigas during April 2018-January 2019 were investigated by 16 S rRNA gene sequencing.The microbiota in C.gigas exhibited tissue heterogeneity,of which Spirochaetaceae was dominant in the gill and hemolymph while Mycoplasmataceae enriched in the hepatopancreas.Co-occurrence network demonstrated that the gill microbiota exhibited higher inter-taxon connectivity while the hemolymph microbiota had more modules.The richness(Chao 1 index)and diversity(Shannon index)of microbial community in each tissue showed no significant seasonal variations,except for the hepatopancreas having a higher richness in the autumn.Similarly,beta diversity analysis indicated a relatively stable microbiota in each tissue during the sampling period,showing relative abundance of the dominant taxa exhibiting temporal dynamics.Results indicate that the microbial community in C.gigas showed a tissue-specific stability with temporal dynamics in the composition,which might be essential for the tissue functioning and environmental adaption in oysters.This work provides a baseline microbiota in C.gigas and is helpful for the understanding of host-microbiota interaction in oysters.

关 键 词：Pacific oyster associated microbiota spatial compartmentalization temporal stability 

分 类 号：S917.1[农业科学—水产科学]