An Improved Chromosome-Level Genome Assembly and Annotation of Belted Beard Grunt(Hapalogenys analis)  

作　　者：GAO Tianxiang WANG Yiting SHI Huilai PING Hongling LIU Qi ZHANG Yang 

机构地区：[1]Fishery College,Zhejiang Ocean University,Zhoushan 316022,China [2]Zhejiang Province Key Laboratory of Mariculture and Enhancement,Zhejiang Marine Fisheries Research Institute,Zhoushan 316021,China [3]Wuhan Onemore-Tech Co.,Ltd.,Wuhan 430076,China [4]Shenzhen Institute of Quality&Safety Inspection and Research,Shenzhen 518000,China

出　　处：《Journal of Ocean University of China》2024年第4期1026-1034,共9页中国海洋大学学报（英文版）

基　　金：supported by the Province Key Research and Development Program of Zhejiang (No.2021C02047);the Special Projects of Zhejiang Provincial Science and Technology Department (Nos.HYS-CZ-004,HYS-CZ-202208);the‘San Nong Jiu Fang’Science and Technology Cooperation Project of Zhejiang Province (No.2022 SN JF073)。

摘　　要：Hapalogenys analis(order Lobotiformes)is an economically and ecologically significant fish species.It is a typical sedentary rocky reef fish and is primarily found in the northern Pacific Ocean.Here,we used Hi-C and PacBio sequencing technique to assemble a high-quality,chromosome-level genome for this species.The 539 Mb genome had a contig N50 with a size of 3.43 Mb,while 755 contigs clustered into 24 chromosomal groups with an anchoring rate of 99.02%.Of the total genomic sequence,132.74Mb(24.39%)were annotated as repeat elements.A total of 21360 protein-coding genes were identified,of which 20787 genes(97.32%)were successfully annotated to public databases.The BUSCO evaluation indicated that 96.90%of the total orthologous genes were matched.The phylogenetic tree representing H.analis and 14 other bony fish species indicated that the H.analis genome contained 364 expanded gene families related to olfactory receptor activity,compared with the common ancestor of H.analis and Sciaenidae.Comparative genomic analysis further identified 3584 contracted gene families.Branch-site modeling identified 277 genes experiencing positive selection,which may facilitate the adaptation to rocky reef environments.The genome reported here is helpful for ecological and evolutionary studies of H.analis.

关 键 词：Hapalogenys analis de novo assembly PacBio comparative genomics 

分 类 号：Q953[生物学—动物学]