Combined Effect of Temperature and Cadmium on Molecular Responses of Hsp70 and P-gp Genes in Crassostrea gigas  

作　　者：HU Fanguang SUN Ming LIU Guangbin JING Yuanyuan CHEN Qun ZHANG Tianwen 

机构地区：[1]Marine Science Research Institute of Shandong Province,Qingdao 266104,China

出　　处：《Journal of Ocean University of China》2024年第4期1115-1120,共6页中国海洋大学学报（英文版）

基　　金：supported by the earmarked fund for the Modern Agroindustry Technology Research System in Shandong Province (No.SDAIT-14)。

摘　　要：To evaluate the combined effect of temperature and cadmium on the molecular responses of heat shock protein 70(hsp70)and P-glycoprotein(P-gp)in mantle,digestive gland and gills of Crassostrea gigas,oysters were exposed to combinations of five temperature levels(10,15,20,25,and 30℃)and 10μg L^(-1)cadmium for 21 days.Oysters were sampled for mRNA quantification by qPCR,and the results showed that the P-gp gene expression changed significantly after treatment at different temperatures and different treatment times.The P-gp gene expression was the highest in the digestive gland.Compared with the control group,the P-gp gene expression in cadmium treatment groups at all the different temperatures were significantly higher than the control group.The control oysters(kept at 10℃during the whole experiment without cadmium)expressed low levels of hsp70,but the groups treated with cadmium displayed somewhat higher levels.The present study demonstrated hsp70 and P-gp played an important role in the detoxification of Cd in C.gigas,and confirmed temperature should be considered for the assessment of Cd-induced toxicity in oysters.

关 键 词：temperature CADMIUM P-GP HSP70 Crassostrea gigas 

分 类 号：S917.4[农业科学—水产科学]