高效液相色谱法测定岩黄连药材不同部位脱氢卡维丁的含量  

Determination of dehydrocavir content in different parts of Yanhuanglian medicinal herb by high-performance liquid chromatography

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作  者:吕倩 武琴园 孙鑫 陈子龙 张春娟 Lv Qian;Wu Qin-yuan;Sun Xin;Chen Zi-long;Zhang Chun-juan(Weinan Inspection and Testing Research Institute,Weinan 714000,China)

机构地区:[1]渭南市检验检测研究院,陕西渭南714000

出  处:《北方药学》2024年第7期1-3,共3页Journal of North Pharmacy

摘  要:目的:旨在开发一项高效液相色谱技术,用于检测岩黄连中脱氢卡维丁有效成分的含量。方法:采用Shiseido Spolar C18色谱柱(粒径5μm,规格250mm×4.6mm),流动相为乙腈-0.05moL·L^(-1)磷酸二氢钠溶液=25:75(取6g无水磷酸二氢钠加超纯水配置成1L的溶液),流速可达1.0mL·min^(-1),波长设置为347nm,柱温维持在30℃。结果:岩黄连药材中有效成分脱氢卡维丁在0.0241~0.3856μg(r=0.9999,n=5)范围内呈现良好线性关系,平均回收率为99.48%,RSD=1.44%。根部脱氢卡维丁含量高于其他部位。结论:这项技术快速准确,专属性强,为开发岩黄连药材和质量控制提供技术支撑。Objective:To establish an efficient liquid chromatography method for determining the content of dehydrocavirin,an effective component of Yanhuanglian medicinal herb;Method:Using a Shiseido Spolar C18 chromatography column(particle size 5)μm.Specification:250mm×4.6mm,mobile phase:acetonitrile-0.05mol·L^(-1)sodium dihydrogen phosphate solutio n=25:75(6g anhydrous sodium dihydrogen phosphate was prepared with ultrapure water to form a 1L solution),flow rate:1.0mL·min^(-1),wavelength set at 347nm,column temperature maintained at 30℃.Result:The effective ingredient dehydrocavir in the medicinal herb of Yanhuanglian ranges from 0.0241 to 0.3856μg.There is a good linear relationship within the range of g(r=0.9999,n=5),with an average recovery rate of 99.48%and RSD=1.44%.The content of dehydrocavirin in the roots is higher than that in other parts.Conclusion:This method is fast,accurate,and has strong specificity,providing technical support for the development and quality control of Yanhuanglian medicinal materialsc.

关 键 词:高效液相色谱法 脱氢卡维丁 含量测定 岩黄连 

分 类 号:R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]

 

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