基于p38 MAPK/NF-κB信号通路探究芍药醇对创伤性脑损伤小鼠神经保护作用的机制研究  

Mechanism of neuroprotective effect of paeoniflorol on traumatic brain injury mice based on p38 MAPK/NF-κB signaling pathway

作　　者：姜乔继 杨红伟 丁亮 韦敏 万政强 JIANG Qiaoji;YANG Hongwei;DING Liang;WEI Min;WAN Zhengqiang(Department of Neurosurgery,The Yancheng Clinical College of Xuzhou Medical University,The First people s Hospital of Yancheng,Yancheng 224001,China)

机构地区：[1]徐州医科大学盐城临床学院盐城市第一人民医院神经外科,盐城224001 [2]徐州医科大学附属医院放射科

出　　处：《临床神经外科杂志》2024年第4期371-376,共6页Journal of Clinical Neurosurgery

基　　金：江苏省医学创新中心建设项目(CXZX202212);江苏省研究生科研与实践创新计划课题项目(SJCX23_1405)。

摘　　要：目的 预测芍药醇对创伤性脑损伤(TBI)后神经功能恢复和神经炎症的影响,以评估其在临床实践中的潜在价值。方法 采取控制皮质撞击(CCI)法建立成年小鼠TBI模型,尾静脉注射后评估观测芍药醇的治疗效能。网络药理学预测了p38 MAPK/NF-κB是作为芍药醇治疗TBI的潜在路线。通过蛋白质印迹及酶联免疫吸附试验进行评估目标蛋白质和炎症因子的表达。采用尼氏染色法观察神经元损伤情况。通过改良神经功能缺损评分(mNSS)评价TBI后的神经恢复情况。结果 TBI后给予芍药醇可预防CCI小鼠的神经元死亡,减少神经炎症,促进神经功能恢复。网络药理学研究表明芍药醇可能通过p38 MAPK/NF-κB路径体现其治疗效能。结论 芍药醇给药后在TBI疾病病程发展过程中具有较好的神经保护和抗炎特性。其机制可能与p38 MAPK/NF-κB信号通路的调节有关。Objective To predict the effect of paeoniflorol on neurological function recovery and neuroinflammation after traumatic brain injury(TBI) and evaluate its potential value in clinical practice.Methods A model of TBI in adult mice was established by controlled cortical impingement(CCI) method,and the therapeutic efficacy of paeoniflorol was evaluated after tail vein injection.Network pharmacology predicted that p38 MAPK/NF-κB was a potential route for the treatment of TBI with paeoniflorol.Western blotting and enzyme-linked immunosorbent assay were used to evaluate the expression of target proteins and inflammatory cytokines.Nissl staining was used to observe neuronal damage.Modified Neurological Severity Score(mNSS) was used to evaluate neurological recovery after TBI.Results Paeoniflorol administration after TBI prevented neuronal death,reduced neuroinflammation and promoted neurological recovery in CCI mice.Network pharmacology studies had shown that paeoniflorol may reflect its therapeutic efficacy through the p38 MAPK/NF-κB pathway.Conclusions Paeoniflorol has superior neuroprotective and anti-inflammatory properties during the course of TBI disease development,and its mechanism may be related to the regulation of the p38 MAPK/NF-κB signaling pathway.

关 键 词：芍药醇 创伤性脑损伤 神经功能 炎症 

分 类 号：R651[医药卫生—外科学]