妇科千金方及其拆方对盆腔炎性疾病后遗症大鼠的作用  

Effects of Fuke Qianjin Formula and its modifications on the sequelae of pelvic inflammatory disease in rats

作　　者：徐佳[1] 谭雅莉 肖志葵 匡继林[1] 林丽美[3] 雷磊[4] XU Jia;TAN Ya-li;XIAO Zhi-kui;KUANG Ji-lin;LIN Li-mei;LEI Lei(The Second Hospital Affiliated to Hunan University of Chinese Medicine,Changsha 410005,China;The Third Hospital Affiliated to Hunan University of Chinese Medicine,Zhuzhou 412000,China;School of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China;College of Integrated Traditional Chinese Medicine and Western Medicine,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学第二附属医院,湖南长沙410005 [2]湖南中医药大学第三附属医院,湖南株洲412000 [3]湖南中医药大学药学院,湖南长沙410208 [4]湖南中医药大学中西医结合学院,湖南长沙410208

出　　处：《中成药》2024年第8期2571-2579,共9页Chinese Traditional Patent Medicine

基　　金：国家中药标准化项目(ZYBZH-C-HUN-21);湖南省中医药管理局重点项目(2020011);湖南省自然科学基金(2022JJ80085);湖南中医药大学科研基金项目(2022XYLH027);湖南中医药大学校级研究生创新课题(2021CX52)。

摘　　要：目的研究妇科千金方及其拆方对盆腔炎性疾病后遗症大鼠的作用。方法SD大鼠随机分为正常组、假手术组、模型组、康妇炎组(0.756 g/kg)、妇科千金方组(2.7 g/kg)、抑菌方组(2.7 g/kg)、抗炎方组(2.7 g/kg)、补益方组(2.7 g/kg),灌胃给予相应药物28 d后处死,计算子宫、脾脏、胸腺、肝脏指数,肉眼观察大鼠、输卵管形态学改变,HE染色观察大鼠子宫、输卵管组织病理学变化,ELISA法检测大鼠血清IL-1β、IL-18、IL-6水平,Western blot法检测大鼠子宫组织TGF-β1、CTGF、NLRP3、caspase-1、IL-18蛋白表达,流式细胞术检测大鼠脾脏组织CD4、CD8表达。结果与模型组比较,妇科千金方组、抑菌方组、抗炎方组、补益方组大鼠子宫、脾脏、胸腺指数均降低(P<0.01);妇科千金方组、抗炎方组大鼠子宫肿胀减轻,子宫内膜腺体增多,炎性渗出减轻(P<0.01),血清IL-1β、IL-18、IL-6水平降低(P<0.01);妇科千金方组、抗炎方组、补益组大鼠子宫组织TGF-β1、CTGF蛋白表达降低(P<0.05);妇科千金方组、抗炎方组大鼠子宫组织NLRP3、caspase-1、IL-18蛋白表达降低(P<0.01);妇科千金方组、补益组CD4^(+)/CD8^(+)比值升高(P<0.01)。结论妇科千金方及其拆方对盆腔炎性疾病后遗症大鼠具有保护作用,其抗炎作用机制可能是通过抑制NLRP3小体的活化,从而阻碍caspase-1的活化,降低促炎症因子IL-18的分泌及释放,抑制细胞焦亡而发挥抗炎作用。AIM To study the effects and possible mechanism of Fuke Qianjin Formula and its modifications on sequelae of pelvic inflammatory disease(PID)in rats.METHODS The SD rats were randomly divided into the normal group,the sham operation group,the model group,Kangfuyan group(FYK,0.756 g/kg),Fuke Qianjin Formula group(FKQ,2.7 g/kg),Yijun Formula group(YJ,2.7 g/kg),Kangyan Formula group(KY,2.7 g/kg)and Buyi Formula group(BY,2.7 g/kg).After 28 days of corresponding administration by gavage,the rats were put to death to procure their uterus,spleen,thymus and liver for the calculation of the organs indices levels;the observation of the general morphology of uterus and oviduct and histopathological examination of uterus and oviduct;the measurement of.the serum levels of IL-1β,IL-18 and IL-6 by ELISA;the detection of the uterine expressions of TGF-β1,caspase-1,NLRP3,IL-18 and CTGF by Western blot;and the detection of the expressions of CD4^(+)and CD8^(+)in spleen lymphocytes by flow cytometry.RESULTS Compared with the model group,FKQ and YJ group,KY group and BY group displayed decreased indices levels of the uterus,spleen and thymus(P<0.01);FKQ group and KY group displayed reduced uterine swelling,increased endometrial glands,and reduced inflammatory exudation(P<0.01),and lower serum levels of IL-1β,IL-18 and IL-6(P<0.01);FKQ group,KYgroup and BY group displayed decreased protein expressions of TGF-β1 and CTGF(P<0.05,P<0.01);FKQ group and KY group displayed decreased protein expressions of NLRP3,caspase-1 and IL-18(P<0.05,P<0.01);and FKQ group and BY group displayed increased CD4^(+)/CD8^(+)ratio(P<0.01).CONCLUSION Being protective to sequelae of PID rat models,Fuke Qianjin Formula and its modifications may contribute their anti-inflammatory efficacy by inhibiting the activation of NLRP3 body to hinder the activation of caspase-1,reduce the secretion and release of pro-inflammatory factor IL-18,and thus inhibit pyroptosis.

关 键 词：妇科千金方及其拆方 盆腔炎性疾病后遗症 抗炎 细胞焦亡 

分 类 号：R285.5[医药卫生—中药学]