山橿根大极性部位化学成分及其抗肿瘤活性研究  

Chemical constituents from the large polar fraction of the roots of Lindera reflexa and their antitumor activities

作　　者：田志浩 孙孝亚[1,2] 张珊珊 郜志宁[1,2] 李焱 陈若曦 陈随清[1,2,3] TIAN Zhi-hao;SUN Xiao-ya;ZHANG Shan-shan;GAO Zhi-ning;LI Yan;CHEN Ruo-xi;CHEN Sui-qing(Henan University of Chinese Medicine,Zhengzhou 450046,China;Henan Key Laboratory of Chinese Medicine Resources and Chinese Medicine Chemistry,Zhengzhou 450046,China;Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases co-constructed by Henan Province&Education Ministry of P.R.China,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学,河南郑州450046 [2]河南省中药资源与中药化学重点实验室,河南郑州450046 [3]呼吸疾病中医药防治省部共建协同创新中心,河南郑州450046

出　　处：《中成药》2024年第8期2617-2623,共7页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(81773859);河南省科技攻关项目(212102310353);河南省中药材产业技术体系(豫财科[2023]7号)。

摘　　要：目的研究山橿根大极性部位的化学成分及其抗肿瘤活性。方法山橿根大极性部位采用硅胶柱、Sephadex LH-20凝胶柱、半制备HPLC、ODS中压柱进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用MTT法测定其抗肿瘤活性。结果从中分离得到13个化合物,分别鉴定为2,6-二甲氧基-4-羟基苯基-1-O-β-D-葡萄糖苷(1)、3-羟基-4,5-二甲氧基苯酚-β-D-吡喃葡萄糖苷(2)、紫丁香苷(3)、1-O-3,4-二甲氧基-5-羟苯基-(6-O-3,5-二甲氧基没食子酰)-β-D-吡喃葡萄糖苷(4)、p-cymen-7-ylβ-D-glucopyranoside(5)、pisumionoside(6)、staphylionoside D(7)、dendranthemoside B(8)、lynoiside(9)、nudiposide(10)、淫羊藿次苷B1(11)、(2 S)-pinocembrin-7-O-(6-O-α-L-rhamnopyranosyl-β-D-glucopyranoside)(12)、(+)-N-(methoxycarbonyl)-N-norboldine(13)。化合物3、13对人肺癌细胞(A549)、人胃癌细胞(MGC80-3)表现出明显的细胞毒活性。结论化合物1~13为首次从山橿中分离得到。化合物3、13具有良好的抗肿瘤活性。AIM To study the chemical constituents from the large polar fraction of the roots of Lindera reflexa Hemsl.and their antitumor activities.METHODS The large polar fraction from the roots of L.reflexa was isolated and purified by silica gel column,Sephadex LH-20 gel column,semi-preparative HPLC and ODS medium pressure column,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The antitumor activities were determined by MTT method.RESULTS Thirteen compounds were isolated and identified as 2,6-dimethoxy-4-hydroxyphenyl-1-O-β-D-glucopyranoside(1),3-hydroxy-4,5-dimethoxyphenol-β-D-glucopyranoside(2),syringin(3),1-O-3,4-dimethoxy-5-hydroxyphenyl-(6-O-3,5-dimethoxygalloyl)-β-D-glucopyranoside(4),p-cymen-7-ylβ-D-glucopyranoside(5),pisumionoside(6),staphylionoside D(7),dendranthemoside B(8),lynoiside(9),nudiposide(10),icariside B1(11),(2 S)-pinocembrin-7-O-(6-O-α-L-rhamnopyranosyl-β-D-glucopyranoside)(12),(+)-N-(methoxycarbonyl)-N-norboldine(13).Compounds 3 and 13 showed obvious cytotoxicity against human lung cancer cells(A549)and human gastric cancer cells(MGC80-3).CONCLUSION Compounds 1-13 are isolated from the roots of L.reflexa for the first time.Compounds 3 and 13 have good anti-tumor activities.

关 键 词：山橿 根 化学成分 分离鉴定 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学]