月季响应高温胁迫HSFA类基因的鉴定及其激素和氧化响应分析  

Identification and plant hormones and oxidation response analysis of heat stress response HsFA genes in Rosa chinensis

作　　者：曹嵛鑫 王珂 金禧 李泽卿 邢文 CAO Yuxin;WANG Ke;JIN Xi;LI Zeqing;XING Wen(College of Landscape Architecture,Central South University of Forestry and Technology,Changsha 410004,China;Hunan Province Nature Reserve Scenic Resources Big Data Engineering Technology Research Center,Changsha 410004,China;College of Nuclear Technology and Chemical Biology,Hubei University of Science and Technology,Xianning,Hubei 437000,China)

机构地区：[1]中南林业科技大学风景园林学院,长沙410004 [2]湖南省自然保护地风景资源大数据工程技术研究中心,长沙410004 [3]湖北科技学院核技术与化学生物学院,湖北咸宁437000

出　　处：《植物生理学报》2024年第7期1132-1144,共13页Plant Physiology Journal

基　　金：国家自然科学基金(32372754和31501794);湖南省自然科学基金(2022JJ31004和2021JJ41070);湖南省林业科技攻关与创新资金项目(XLKY202327);湖南省教育厅重点项目(21A0171);长沙市自然科学基金(kq2202273)。

摘　　要：热激转录因子(heat shock transcription factor,HSF)是植物响应高温胁迫的重要调控因子。本研究对月季(Rosa chinensis)的HSF基因家族进行了全面鉴定,在月季基因组中鉴定出18个HSF家族成员,包含A、B、C三类,其中A类11个、B类6个、C类1个。顺式作用元件分析发现月季HSFA基因的启动子序列含有响应不同非生物胁迫的元件,基本都含有响应高温胁迫的元件。在高温胁迫下A1、A2、A3、A4、A5和A7亚家族的6个基因在叶片中显著上调表达。进一步分析发现,在高温胁迫下除A1亚家族基因在花中从处理12 h开始显著上调外,其他5个基因在根和花中短期内就开始显著上调。植物生长调节物质和氧化胁迫分析结果表明6个基因均显著响应脱落酸(ABA)、赤霉素(GA_(3))、茉莉酸甲酯(MeJA)、水杨酸(SA)和过氧化氢(H_(2)O_(2))胁迫。除MeJA以外,这些基因在其他处理下均表现出不同的表达模式;在氧化胁迫下除A5亚家族的RcHsf9外,其他5个基因均为短期响应。本研究为进一步研究月季HSFA转录因子的功能和调控机制奠定了基础。HsF transcription factors are the important regulators in heat stress response in plants.In this study,we comprehensively analyzed the HSF gene family in the Rosa chinensis genome and 18 HSF family members were identified.These HSFs can divided into three classes,A,B and C.11,6 and 1 members be-longed to class A,B and C,respectively.Cis-acting element analysis revealed that the promoter of RcHSFA harbored different abiotic stresses elements,and most of them contained elements that response to heat stress.Six genes of subfamilies A1,A2,A3,A4,A5 and A7 were significantly up-regulated in leaves under heat stress treatment.Furthermore,among these genes,five genes were significantly up-regulated in both roots and flowers by heat stress in the short time.One member of A1 subfamily was significantly up-regulated at 12 h in flowers.All six genes were significantly induced by abscisic acid(ABA),gibberellic acid(GA_(3)),methyl jasmonate(MeJA),salicylic acid(SA)and hydrogen peroxide(H_(2)O_(2))stress.Except for MeJA,six genes displayed distinct expression patterns in response to other hormone treatments.And besides RcHsf9 of A5 subfamily,other five genes exhibited a rapid response to oxidative stress.These results established the fundamental basis for further studies on the function and regulatory mechanism of HSFA transcription factors in R.chinensis.

关 键 词：月季 高温 热激转录因子 激素 氧化胁迫 

分 类 号：S685.12[农业科学—观赏园艺]