64份醋栗番茄核心种质萌发期耐盐碱评价  

Evaluation of saline-alkali tolerance of 64 core germplasms of Solanum pimpinellifolium at germination stage

作　　者：孙明明 郭冉博 李鑫 刘磊[2] 朱璨 黄泽军[2] 王孝宣[2] 国艳梅[2] 杜永臣[2] 朱文莹 李君明[2] SUN Mingming;GUO Ranbo;LI Xin;LIU Lei;ZHU Can;HUANG Zejun;WANG Xiaoxuan;GUO Yanmei;DU Yongchen;ZHU Wenying;LI Junming(College of Horticulture,Qingdao Agricultural University,Qingdao,Shandong 266109,China;State Key Laboratory of Vegetable Biobreeding,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]青岛农业大学园艺学院,山东青岛266109 [2]中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,北京100081

出　　处：《植物生理学报》2024年第7期1145-1156,共12页Plant Physiology Journal

基　　金：国家自然科学基金(31991185);农业农村部园艺作物生物学与种质创制重点实验室项目(2018YFD1000801);中国农业科学院科技创新工程项目(CAASASTIP-IVFCAAS)。

摘　　要：土壤盐碱化严重影响作物生产。野生种在适应外界环境的过程中,演化出较好的应对非生物胁迫的能力。鉴于当前在耐盐碱资源挖掘方面存在的不足,本研究对收集的野生醋栗番茄核心种质在Na_(2)CO_(3)胁迫条件下的萌发特性进行了评价。结果表明:Na_(2)CO_(3)溶液对番茄种子的萌发具有明显的抑制作用,但不同资源对Na_(2)CO_(3)胁迫的耐受性存在显著差异。在60 mmol·L^(-1)的高Na_(2)CO_(3)浓度条件下,综合相对发芽势、相对发芽率与相对发芽指数,共鉴定出P8、P9、P12、P13、P25、P30、P40、P50、P51、P53、P56、P61、P62和P63等14份耐盐碱材料与22份盐碱敏感材料,耐盐碱材料相对发芽率与相对发芽势可达85%以上,其中P51与P61相对发芽率与相对发芽势达到100%,具有极强的耐盐碱能力。通过全基因组数据及已报道的来自醋栗番茄的耐盐碱SlScaBP8基因变异分析,推测这些资源的耐盐碱性状由不同基因控制,一些资源包含耐盐碱新位点,且耐盐和耐碱可能存在不同的调控机制。研究结果为利用野生资源进行耐盐碱遗传改良和机制解析提供了新依据。Soil salinization seriously affects crop production.Wild species have evolved a better ability to cope with abiotic stress in the process of adapting to the external environment.In view of the current defi-ciencies in the excavation of saline-alkali tolerant resources,the germination characteristics of wild Sola-num pimpinellifolium core collection under Na_(2)CO_(3)stress were evaluated in this study.The results showed thatNa_(2)CO_(3)solution had a significant inhibitory effect on the germination of tomato seeds,but the toler-ance of different resources to Na_(2)CO_(3)stress was significantly different.Under the condition of high Na_(2)CO_(3)concentration of 60 mmol·L^(-1),the relative germination potential,relative germination rate and relative germination index were comprehensively identified.A total of 14 saline-alkali tolerant materials such as P8,P9,P12,P13,P25,P30,P40,P50,P51,P53,P56,P61,P62 and P63 and 22 saline-alkali sensitive materials were identified.The relative germination rate and relative germination potential of saline-alkali tolerant materials could reach more than 85%.Among them,the relative germination rate and relative germination potential of P51 and P61 reached 100%,which had strong saline-alkali tolerance.Through the analysis of genome-wide data and the reported salt-tolerant SIScaBP8 gene variation from currant tomatoes,it is speculated that the salt-tolerant traits of these resources are controlled by different genes,and some resources contain new salt-tolerant sites,and salt-tolerant and alkali-tolerant may have different regulatory mechanisms.The results of this study provide a new basis for genetic improvement and mechanism analysis of saline-alkali tolerance using wild resources.

关 键 词：Na_(2)CO_(3) 醋栗番茄 耐盐碱 萌发期 评价 

分 类 号：S641.2[农业科学—蔬菜学]