浙江豇豆地方品种的遗传多样性  

Genetic diversity of cowpea landraces in Zhejiang Province,China

作　　者：汪宝根[1,2] 陈小央 吴健 李潇[1,4] 汪颖 王尖 吴晓花 鲁忠富[1,2] 孙玉燕[1,2] 董文其[1,2] 李国景[1,2] 吴新义[1,2,5] WANG Baogen;CHEN Xiaoyang;WU Jian;LI Xiao;WANG Ying;WANG Jian;WU Xiaohua;LU Zhongfu;SUN Yuyan;DONG Wenqi;LI Guojing;WU Xinyi(Institute of Vegetable,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China(Co-Construction by Ministry and Province),Ministry of Agriculture and Rural Affairs,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;General Station for Seed Administration of Zhejiang Province,Hangzhou 310020,China;College of Horticulture Science,Zhejiang A&F University,Hangzhou 311300,China;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]浙江省农业科学院蔬菜研究所,浙江杭州310021 [2]浙江省农业科学院农业农村部南方豆类蔬菜种质创新与分子育种重点实验室(部省共建),浙江杭州310021 [3]浙江省种子管理总站,浙江杭州310020 [4]浙江农林大学园艺科学学院,浙江杭州311300 [5]浙江省农业科学院省部共建农产品质量安全危害因子与风险防控国家重点实验室,浙江杭州310021

出　　处：《浙江农业学报》2024年第7期1569-1582,共14页Acta Agriculturae Zhejiangensis

基　　金：2022-2024年农作物种质资源精准鉴定评价任务(豇豆)(2023R23T60D01,2022R23T60D04);浙江省农业新品种选育重大专项(2021C02065-6-3);浙江省重点研发计划(2022C02016)。

摘　　要：浙江省是我国豇豆的主要产区之一,为全面梳理、鉴定浙江省地方豇豆种质资源,对浙江省历年来收集到的220份豇豆地方品种进行21个重要性状的田间鉴定。结果表明,浙江省豇豆地方种质具有丰富的表型变异,基于表型数据的聚类分析可以将它们分为4个类群,其中类群Ⅲ的综合表现接近目前生产上的育成品种,具有较高的育种使用价值。本研究还利用豇豆物种特异的核心单核苷酸多态性(SNP)标记对其进行基因型鉴定,构建了各种质DNA指纹图谱,基于基因型数据将之分为4个亚群,其中亚群Ⅲ的荚长较长。本研究从多角度评价了浙江省豇豆地方种质的遗传多样性,研究结果可以为豇豆种质创新和新品种选育提供科学依据。Zhejiang is one of the main cowpea producing areas in China.To systematically sort out and identify the cowpea landraces in Zhejiang Province,220 cowpea accessions collected in Zhejiang Province in multi-years were evaluated on 21 important traits in the field.The results showed that these landraces had large phenotypic variation,and they could be divided into four clusters according to their phenotype data,of which,ClusterⅢdisplayed the similar phenotypes with the current released cultivars,indicating that these accessions had high values for cowpea breeding.Using the cowpea specific core single nucleotide polymorphisms(SNPs),the genotypes of 220 landraces were also identified and their DNA fingerprinting was constructed.Based on their genotype data,these accessions were also divided into four subpopulations,and subpopulationⅢshowed the longest pods.In summary,the genetic diversity of Zhejiang cowpea landraces was evaluated from different perspectives,and the results would provide a scientific basis for cowpea germplasm enhancement and new cultivars development.

关 键 词：豇豆 种质资源 遗传多样性 基因型 

分 类 号：S643.4[农业科学—蔬菜学]