六十份甘薯种质资源对基腐病的抗性评价与抗病相关指标分析  

Evaluation of resistance to foot rot and analysis of disease resistance related indexes of 60 sweet potato germplasm resources

作　　者：韩清宇 程林润 李月红 仇智灵 后猛[5] 楼兵干[1] HAN Qingyu;CHENG Linrun;LI Yuehong;QIU Zhiling;HOU Meng;LOU Binggan(Institute of Biotechnology,Zhejiang University,Hangzhou 310058,China;Jinhua Academy of Agricultural Sciences,Jinhua 321017,Zhejiang,China;Plant Protection Station of Jinhua City,Jinhua,321017,Zhejiang,China;Hangzhou Lin an Plant Quarantine Station,Hangzhou 311300,China;Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District,Xuzhou 221131,Jiangsu,China)

机构地区：[1]浙江大学生物技术研究所,浙江杭州310058 [2]金华市农业科学研究院,浙江金华321017 [3]金华市植物保护站,浙江金华321017 [4]杭州市临安区植物检疫站,浙江杭州311300 [5]江苏徐淮地区徐州农业科学研究所,江苏徐州221131

出　　处：《浙江农业学报》2024年第7期1616-1625,共10页Acta Agriculturae Zhejiangensis

摘　　要：近年来甘薯基腐病是浙江甘薯生产上发生的重大病害,严重影响了甘薯的产量与品质。本研究旨在发掘高抗基腐病的甘薯栽培品种,并解析抗性相关的生理指标以及抗病相关基因表达特征,为甘薯品种改良提供科学依据。本文采用伤口接种法,对60份不同甘薯种质资源进行了基腐病抗性评价,并分析了抗、感病甘薯品种接种基腐病病原菌后抗氧化酶活性、丙二醛(MDA)含量以及抗病相关基因表达水平的差异。结果表明,60份参试甘薯材料中,仅3份表现为高抗甘薯基腐病,分别为徐D1-26、徐D10-139、J7-44。10份表现为抗病、18份表现为感病、29份表现为高感。甘薯对基腐病病原菌的抗性水平与甘薯抗氧化能力、膜脂过氧化水平以及茉莉酸信号途径调控有关。接种甘薯基腐病病原菌后,高抗品种的过氧化物酶(POD)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性明显高于高感品种,接种第120 h,高抗品种POD和SOD活性分别是高感品种的1.81倍和3.75倍,接种后48 h,高抗品种CAT活性是高感品种的2.93倍,而高抗品种MDA含量显著低于高感品种;qRT-PCR分析表明,茉莉酸信号途径调控的抗病相关基因IbWRKY 1、IbBBX 24和IbNAC 1在高抗品种中均表达上调,接种后168 h,其表达量分别是高感品种的2.72倍、3.15倍和1.91倍。Foot rot of sweet potato is the main disease of sweet potato in Zhejiang Province in recent years,which seriously affects the yield and quality of sweet potato.The purpose of this study was to explore sweet potato varieties with high resistance and to explore the characteristics of resistance-related physiological indexes and resistance-related genes,so as to provide scientific basis for the improvement of sweet potato varieties.The resistance to foot rot of 60 sweet potato germplasm resources was determined by wound inoculation method.The representative resistant and susceptible sweet potato varieties were selected to infect the Phomopsis destruens and the differences in the activities of antioxidant enzymes,malondialdehyde(MDA)content and the expression levels of resistance-related genes were analyzed.The results showed that among the 60 sweet potato germplasm resources,3 varieties were highly resistant to the disease,including Xu D1-26,Xu D10-139,and J7-44.Meanwhile,10 resistant,18 susceptible and 29 highly susceptible varieties were identified,respectively.Moreover,the resistance level of sweet potato to foot rot was related to the antioxidant capacity,membrane lipid peroxidation level and jasmonic acid signaling pathway regulation of sweet potato.We found that the activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)in highly resistant varieties were significantly increased compared to highly susceptible varieties.By 120 hours post inoculation(hpi),the activities of POD and SOD in highly resistant varieties were 1.81 times and 3.75 times higher than those in highly susceptible varieties,and the activity of CAT in highly resistant varieties was 2.93 times higher by 48 hpi.However,the MDA content of highly resistant varieties was significantly lower than that of highly susceptible varieties.Quantitative RT-PCR analysis showed that expression levels of the resistance-related genes IbWRKY1,IbBBX 24 and IbNAC 1 were up-regulated by 2.72,3.15 and 1.91 times in highly resistant varieties compared

关 键 词：甘薯 种质资源 甘薯基腐病病原菌 抗性评价 抗性指标 

分 类 号：S531[农业科学—作物学]