香樟NAC基因家族的全基因组鉴定及盐胁迫下的表达分析  

Genome-wide identification and expression analysis of NAC gene family under salt stress in Cinnamomum camphora

作　　者：曾伟伟 李一凡 朱渊铭 林宇清 陈丝雨 邹双全[1,2] ZENC Weiwei;LI Yi-fan;ZHU Yuan-ming;LIN Yu-qing;CHEN Si-yu;ZHOU Shuang-quan(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Engineering Research Institute of Conservation,Utilization of Natural Bioresources,Fuzhou 350002,China)

机构地区：[1]福建农林大学林学院,福州350002 [2]自然生物资源保育利用福建省高校工程研究中心,福州350002

出　　处：《西南农业学报》2024年第7期1393-1408,共16页Southwest China Journal of Agricultural Sciences

基　　金：福建省林业科技计划项目(2024FKJ25、2024TG08)。

摘　　要：[目的]探究NAC基因在香樟(Cinnamomum camphora)生长发育及其在盐胁迫下发挥的重要作用,为进一步阐明香樟在盐胁迫下的响应机理奠定基础。[方法]对香樟全基因组NAC基因进行鉴定和生物信息学分析,探究香樟非生物胁迫的响应模式。通过对香樟NAC家族成员进行鉴定,并从理化性质、系统进化分析、保守基序、基因结构、染色体定位、物种间共线性、顺式作用元件预测和表达模式等方面进行系统分析。[结果]共鉴定出103个香樟NAC基因,氨基酸数量介于137~1136个,等电点在4.54~10.00,相对分子质量在15644.40~129859.29 u,平均疏水性全部为负值,均为亲水蛋白,亚细胞定位预测定位在细胞核和细胞质中;通过与拟南芥(Arabidopsis thaliana)NAC基因序列构建系统发育进化树发现,香樟NAC基因被划分为16个亚家族,其中NAM亚家族成员最多,有17个香樟NAC基因,还有25个香樟NAC基因未参与分组;保守基序和基因结构分析发现,香樟NAC基因包含有2~8个内含子和10个motif,其中motif3在香樟基因遗传进化中趋于稳定,motif9和motif10多数分布在NAM亚家族中;染色体定位和复制事件分析发现,103个NAC基因分布在12条染色体中,基因分布最多的是Chr02号染色体(13个CcNAC基因),最少的是Chr10和Chr11(各2个基因),片段复制为香樟NAC家族的主要扩增方式;多物种共线分析发现,香樟与牛樟(Cinnamomum kanehirae)NAC基因形成162对共线基因,多于其它物种,说明香樟NAC基因与牛樟之间的同源进化关系比其他物种更密切;香樟NAC基因启动子中含有多个非生物胁迫、生长发育和激素类响应元件;qRT-PCR结果表明,在叶中,CcNAC058和CcNAC092基因的表达水平在低盐胁迫下达到最大。在根中,CcNAC007、CcNAC033、CcNAC058和CcNAC092基因的表达随着盐浓度的增加而增加。[结论]NAC基因家族在香樟的生长发育进程中可能参与盐胁迫响应过程,在盐胁迫下CcNAC092、CcNAC05[Objective]The present paper aimed to investigate the important role of NAC gene in the growth and development of Cinnamomum camphora and its role in salt stress,and to clarify the response mechanism of C.camphora under salt stress.[Method]The genome-wide NAC genes of C.camphora were identified and analyzed by bioinformatics to investigate the mode of response to abiotic stress in C.campho-ra.By identifying the members of NAC family of C.camphora,the paper aonducteda systematic analysis in terms of physicochemical proper-ties,phylogenetic analysis,conserved motifs and gene structures,chromosomal localization and inter-species covariance,cis-acting element prediction and expression patterns.[Result]A total of 103 CcNAC genes were identified,the amino acid counts ranged from 137 to 1136,the isoelectric points from 4.54 to 10.00,the relative molecular masses from 15644.40 to 129859.29 u,and the mean hydrophobicity of all proteins was negative,indicating hydrophilicity.Subcellular localization was predicted to be in the nucleus and cytoplasm.A phylogenetic evolutionary tree was constructed with Arabidopsis thaliana NAC gene sequences,which classified CcNAC genes into 16 subfamilies.The NAM subfamily had the most members,with 17 CcNAC genes,while 25 CcNAC genes were not included.Conservative motif and gene struc-ture analysis revealed that CcNAC genes were found to contain 2-8 introns and 10 motifs.Among these,motif3 appeared to be particularly stable in the genetic evolution of C.camphora genes,while motif9 and motifl0 were distributed across the NAM subfamily.Chromosome lo-calization and replication event analysis revealed that 103 genes were distributed across 12 chromosomes,with the highest number of genes(13)found in CcNAC genes distributed across chromosomes Chr02,with the lowest number of genes found on Chr10 and Chrl1,which con-tained two genes each;Fragment duplication was the main mode of amplification for the CcNAC family.Muli-species co-linkage analysis re-vealed that CcNAC genes formed 162 pairs of co-lin

关 键 词：香樟 NAC基因家族 盐胁迫 全基因组分析 

分 类 号：S792[农业科学—林木遗传育种]