不同品种烟草患黑胫病根际土壤真菌群落结构研究  

Rhizosphere soil fungal community structure of different varieties of tobacco with black shank disease

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作  者:程凌寒 张裕棠 方宇 刘玲玲 蔡永占 刘冬梅[3] 韩小女[4] 余磊[1] 齐颖 刘佳妮[1] CHENG Ling-han;ZHANG Yu-tang;FANG Yu;LIU Ling-ling;CAI Yong-zhan;LIU Dong-mei;HAN Xiao-nv;YU Lei;QI Ying;LIU Jia-ni(Kunming University/Yunnan Urban Agricultural Engineering and Technological Research Center,Kunming 650214,China;Hefei Gao'er Life and Health Science Research Institute Co.,Ltd.,Hefei 230088,China;Qujing Company,Yunnan Tobacco Company,Qujing,Yunnan 655002,China;Agricultural Technique Popularization Centre of Xuanwei City,Xuanwei,Yunnan 655499,China)

机构地区:[1]昆明学院/云南省都市特色农业工程技术研究中心,昆明650214 [2]合肥高尔生命健康科学研究院有限公司,合肥230088 [3]云南省烟草公司曲靖市公司,云南曲靖655002 [4]云南省宣威市农业技术推广中心,云南宣威655499

出  处:《西南农业学报》2024年第7期1494-1503,共10页Southwest China Journal of Agricultural Sciences

基  金:云南省科技厅联合专项重点项目(2019FH001-008);中国烟草总公司云南省公司科技项目(2020530000242010)。

摘  要:[目的]揭示不同品种患病根际土壤真菌中的差异显著物种,探讨根际土壤真菌与不同品种烟草互作关系,为品种抗性机理研究以及生物防治提供参考。[方法]采用Illumina MiSeq高通量测序技术,分析云南省8个主栽烟草品种的患病植株根际土壤真菌的群落结构组成与多样性。[结果]8个品种的患病根际土壤真菌OTU丰度值分布在822~1126,各品种间根际土壤真菌多样性与丰富度指数存在显著差异,其中品种K326、云105、云87和云99显著高于其他品种。在门水平上,各品种患病根际土壤真菌主要以子囊菌门(Ascomycota)、被孢霉门(Mortierellomycota)和担子菌门(Basidiomycota)为优势菌门。在属水平上,各处理组主要以Microidium属、镰孢菌属(Fusarium)、腐质霉属(Humicola)、被孢霉属(Mortierella)和链格孢属(Alternaria)等为优势菌属。LEFSe显著差异分析表明:腐质霉属、假丝酵母属(Candida)、小不整球壳属(Plectosphaerella)、链格孢属、丝葚霉属(Papulaspora)、镰孢菌属、Boeremia属、被孢霉属、赤霉菌属(Gibberella)是不同品种烟草患病根际土壤的重要差异显著物种。[结论]不同品种烟草的患病根际土壤真菌群落结构与多样性存在显著差异,且显著差异物种的分布与品种抗性存在一定联系。对黑胫病具有较高抗性的品种(云97、K326、云100和云87)中以有益菌属为主,而在感病和中抗品种(红大、云105、云116和云99)中则聚集了部分病原菌。[Objective]The paper aimed to explore the interaction between rhizosphere soil fungi and different tobacco varieties,and reveal significant dfferences in species among different varieties of diseased rhizosphere soil fungi,as to provide reference for the study of resistance mechanism of tobacco varieties and biological pest control.[Method]Illumina MiSeq high-throughput sequencing technology was used to an-alyze the composition and diversity of fungal community in rhizosphere soil of diseased plants of 8 tobacco varieties in Yunnan province.[Re-sult]The OTU abundance of fungi in diseased rhizosphere soil of different varieties ranged from 822 to 1126.There were significant differ-ences in the diversity and richness index of rhizosphere soil fungi among different varieties,and varieties K326,Yun 105,Yun 87 and Yun 99 were significantly higher than other varieties.At phylum level,the dominant fungi in the diseased rhizosphere soil of each variety were Ascomycota,Mortierellomycota and Basidiomycota.At the genus level,Microidium,Fusarium,Humicola,Mortierella and Alternaria were the dominant genera in each treatment group.The significant difference analysis of LEFSe showed that Humicola,Candida,Plectospha-erella,Alternaria,Papulaspora,Fusarium,Boeremia,Mortierella,Gibberella were the important significantly different species in the dis-eased rhizosphere soil of different varieties.[Conclusion]There are significant differences in fungal community structure and diversity in rhi-zosphere soil of different varieties,and the distribution of significantly different species is related to the resistance of varieties.Among the va-rieties with high resistance to black shank disease(Yun 97,K326,Yun 100 and Yun 87),beneficial bacteria are mainly present,while in susceptible and moderately resistant varieties(Hongda,Yun 105,Yun 116 and Yun 99),harmful bacterial groups such as pathogenic bacteria are clustered.

关 键 词:烟草黑胫病 品种 根际土壤 真菌群落 

分 类 号:S154.3[农业科学—土壤学]

 

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