GABA和BABA诱导辣椒幼苗及果实对炭疽病抗性的生理影响  

Physiological effects on pepper seedlings and fruits induced by GABA and BABA to anthrax disease

作　　者：巩雪峰[1,2] 宋占锋 许艺[1,2] 文熙宸 陈鑫 侯思皓 李红 陈琼 GONG Xue-feng;SONG Zhan-feng;XU Yi;WEN Xi-chen;CHEN Xin;HOU Si-hao;LI Hong;CHEN Qiong(Horticulture Institute,Sichuan Academy of Agricultural Sciences/Vegetable Germplasm Innovation and Variety Improvement Key Laboratory of Sichuan Province/Key Laboratory of Horticultural Crop Biology and Germplasm Enhancement in Southwest Reigns,Ministry of Agriculture in Rural Affairs,Chengdu 610066,China;Sichuan Province Engineering Technology Research Center of Vegetables,Chengdu 611934,China)

机构地区：[1]四川省农业科学院园艺研究所/蔬菜种质与品种创新四川省重点实验室/农业农村部西南地区园艺作物生物与种质创制重点实验室,成都610066 [2]四川省蔬菜工程技术中心,成都611934

出　　处：《西南农业学报》2024年第7期1553-1562,共10页Southwest China Journal of Agricultural Sciences

基　　金：四川省科技计划青年创新团队(2021JDT0006);“十四五”四川省蔬菜育种公关项目(2021YFYZ0022);四川省自然科学基金青年基金项目(2023NSFSC1242);国家现代农业产业技术体系四川省创新团队(sccxtd-2020-05);四川省财政自主创新专项项目(2022ZZCX45)。

摘　　要：[目的]研究γ-氨基丁酸(GABA)和β-氨基丁酸(BABA)诱导辣椒幼苗和果实对炭疽病抗性的生理作用。[方法]以辣椒品种‘红冠3号’和‘川腾10号’为试材,采用50 mmol/L GABA和50 mmol/L BABA预处理1/2 MS固体培养基培养的辣椒幼苗和离体果实,并以不添加GABA和BABA为无预处理对照(CK),分析喷雾和针刺法接种炭疽菌(Colletotrichum socvillei)及无菌水(对照)后辣椒幼苗和果实的生理变化。[结果]接种炭疽菌0~8 d,2个辣椒品种幼苗叶片叶绿素a、b均呈下降趋势,在8 d时较对照(无菌水)差异均达显著水平(P<0.05,下同);根系生长速率也持续下降;而GABA和BABA各预处理组的叶绿素含量和根长比无预处理组下降趋势均明显减缓,GABA预处理叶绿素含量均高于其它处理组,在2 d时达显著水平;GABA和BABA各预处理组幼苗抗氧化酶活性均高于无预处理组0 d时,预处理组CAT和SOD活性已在0 d时达到无预处理组接菌8 d时活性水平,MDA含量无明显变化。接种8 d时,GABA预处理幼苗组内接菌8 d的SOD活性显著高于0 d对照,BABA预处理幼苗组内接菌8 d的SOD和POD均显著高于0 d对照;辣椒红果预处理组的抗氧化酶活性均高于无预处理组0 d时活性,预处理组内接菌8 d的CAT和POD活性高于对照0 d,MDA含量无明显变化,且预处理较无预处理接菌8 d红果病斑直径显著降低,分别降低1.34和0.68倍。此外,GABA、BABA预处理果实各对组内的对照0 d和接种8 d及组间的抗氧化酶活性变化和MDA活性变化与幼苗预处理试验结果变化趋势一致。[结论]GABA和BABA预处理提高了接种炭疽菌后辣椒幼苗的叶片叶绿素含量和根系生长速率。GABA和BABA预处理缓解了炭疽菌对辣椒红果的侵染。同时,GABA和BABA通过提高辣椒幼苗和果实抗氧化酶活性,降低MDA的积累,从而提高辣椒幼苗和果实对炭疽病的抗病性。[Objective]The study aimed to investigate the effect ofγ-amino-butyric acid(GABA)andβ-amino-butyric acid(BABA)on pepper seedlings and pepper fruits.[Method]Hongguan No.3'and‘Chuanteng No.10'pepper cultivars were utilized as test materials.Pepper seedlings were cultured in 1/2 MS solid medium,and pre-treated with 50 mmol/L GABA and 50 mmol/L BABA(GABA and BABA were not added as non-pre-treatment control)to analyze physiological changes after inoculation with Colletotrichum socvillei(inoculated with H O as non-inoculation control).Meanwhile,red fruit of peppers were pretreated with 50 mmol/L GABA and 50 mmol/L BABA to analyze the physiological changes after C.socvllei.[Result]After inoculation C.socvllei,the leaves of both‘Hongguan No.3'and‘Chuanteng No.10'pepper seedlings exhibited a decreasing level in chlorophyll a and b as days past inoculations.This decline was significant when compared to the non-inoculated control on 8-day(H_(2)O).(P<0.05,the same as below).The root growth rate of these two pepper cultivars also showed downtrends with days past inoculation;Both GABA and BABA pre-treatment groups showed a reduced decline in chlorophyll content and root length compared to the non-pre-treatment control group after inoculation with C.socvillei,separately.The chlorophyll con-tent of GABA pre-treatment group remained consistently higher than that of the other treatment groups,with a significant dfference observed at 2-day past inoculation.At 8 days past inoculation,the POD activities of GABA and BABA pre-treatment groups were significantly lower than those of the non-pre-treatment control group separately.Moreover,the MDA contents of GABA and BABA pre-treatment groups were significantly lower than that of the non-pre-treatment control group.Furthermore,there were minimal significant differences within each GA-BA and BABA pre-treatment group concerning antioxidant enzymes or MDA levels when comparing from O-day to 8-day past inoculation.However,the antioxidant enzymes activities of GABA and BABA pre-treatme

关 键 词：辣椒 Γ-氨基丁酸 Β-氨基丁酸 炭疽病 辣椒幼苗 

分 类 号：S641.3[农业科学—蔬菜学]