中国新记录属安卵蜂属及2新记录种记述(膜翅目:缘腹细蜂科)  

Description of a newly recorded genus Anteris with two newly recorded species in China(Hymenoptera:Scelionidae)

作　　者：王立 胡红英 康宁 席欧彦 WANG Li;HU Hong-ying;KANG Ning;XI Ou-yan(College of Life Science and Technology,Xinjiang University/Key Laboratory of Biological Resources Genetic Engineering,Urumqi 830046,China)

机构地区：[1]新疆大学生命科学与技术学院/新疆生物资源基因工程重点实验室,乌鲁木齐830046

出　　处：《西南农业学报》2024年第7期1635-1644,共10页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31860612);第三次新疆综合科学考察项目(2021xjkk0503)。

摘　　要：[目的]通过传统形态分类结合DNA条形码方法对重要天敌昆虫安卵蜂属(Anteris)进行分类研究,查明安卵蜂属在新疆伊犁河谷西天山野果林的资源情况,扩增其在NCBI的分子数据,为后续安卵蜂属的快速准确鉴定以及生物学、分子生物学和生态学等研究奠定基础。[方法]整理2021-2023年在我国新疆伊犁河谷西天山野果林通过网扫和黄盘诱集获得的缘腹细蜂科标本,在数码体视显微镜下进行形态特征观察,挑选出安卵蜂属物种,在传统形态鉴定的基础上,利用无损伤提取法提取物种基因组DNA,用通用引物扩增COI序列和28S rDNA序列,将其序列作为物种的DNA条形码,并与NCBI网站上其他缘腹细蜂科物种COI序列分析比对,分析其碱基组成并计算其遗传距离;基于最大似然法构建系统发育树,进行系统发育分析。[结果]发现一个中国新记录属--安卵蜂属(Anteris)及两个中国新记录种--拟安卵蜂(Anterissimulans)、塞式安卵蜂(A.szelényii),提供新记录属、种形态描述及形态特征图,获得两个新记录种的COI和28S rDNA序列,其中拟安卵蜂的种内遗传距离约0.000~0.003,拟安卵蜂与塞式安卵蜂、A.bilineata的种间遗传距离分别为0.092和0.019,均大于拟安卵蜂的种内最大遗传距离(0.003),拟安卵蜂与A.bilineata亲缘关系更近。[结论]本研究新发现的拟安卵蜂与塞式安卵蜂,进一步丰富了我国及世界缘腹细蜂的资源数据,对缘腹细蜂科类群的保护和利用具有重要意义。[Objective]The traditional morphological classification combined with the DNA barcode method was used to classify the important natural enemies of the genus Anteris,to identify the resources of the genus(Anteris)in the wild fruit forests of the Xitianshan Mountains in the Ili River Valley of Xinjiang,and to amplify its molecular data in NCBI,so as to facilitate the rapid and accurate identification of subse-quent studies,and to lay the foundation for the studies on the biology,molecular biology and ecology of the genus Anteris.[Method]Speci-mens of the family Scelionidae obtained by net sweeping and yellow disc trapping in the wild fruit forests of Xitianshan Mountain,Ili River Valley,Xinjiang,China,in 2021-2023 were collated,and morphological characteristics were observed under a digital stereomicroscope and species of the genus Anteris were selected.On the basis of traditional morphometrics identification,the genomic DNA of the species was extracted by using a non-destructive extraction method,and the genes were amplified using universal primers.COI and 28S rDNA sequences were amplified with universal primers,which were used as DNA barcodes of the species,and compared with the COI sequences of other spe-cies in the family of Scelionidae on the NCBI website,to analyze the base composition and calculate the genetic distances;A phylogenetic tree based on the maximum likelihood method was constructed,and preliminary phylogenetic analyses were carried out.[Result]We found one new Chinese genus-Anteris,and two new Chinese species-Anteris simulans and Anteris szelenyi,and provided morphological de-scriptions of the new genera and species,as well as morphological character pictures We obtained COI and 28S rDNA sequences of the two new species.It could be seen that the intraspecific genetic distances of A.simulans were in the range of 0.000-0.003,and the interspecif-ic genetic distances between A.simulans and A.szelenyi with A.bilineata were 0.095 and 0.019,respectively,which were both smaller than the maximum intraspec

关 键 词：中国新记录属、种 安卵蜂属 DNA条形码 寄生性天敌昆虫 

分 类 号：S893.1[农业科学—特种经济动物饲养]