脑脊液宏基因组学二代测序协助诊断成人重型流行性乙型脑炎1例  

Metagenomic next-generation sequencing of cerebrospinal fluid assisting in the diagnosis of adult severe epidemic encephalitis B:a case report

作　　者：杨佳莉 金钧[1] 李东蓉[1] 俞喆珺 杨新静[1] Yang Jiali;Jin Jun;Li Dongrong;Yu Zhejun;Yang Xinjing(Department of Critical Care Medicine,the First Affiliated Hospital of Soochow University,Suzhou 215006,China)

机构地区：[1]苏州大学附属第一医院重症医学科,苏州215006

出　　处：《中华神经科杂志》2024年第8期890-893,共4页Chinese Journal of Neurology

摘　　要：流行性乙型脑炎病毒感染后临床表现往往为急性脑炎,病原学诊断是常见的诊断方法,但临床上病毒检测、分离比较困难,检出病原体种类比较局限。宏基因组学二代测序(mNGS)是一种快速精准的分子诊断方法,可提高病原微生物的检出率及检出种类,可检测到中枢神经系统感染中不常见的病原体。文中报道1例27岁男性患者,以恶心呕吐、高热、意识障碍及多器官功能衰竭为主要临床表现,临床考虑流行性乙型脑炎,但缺乏病原学证据,遂行脑脊液mNGS检测出乙型脑炎病毒,大大提高了流行性乙型脑炎病毒诊断的精准性。The clinical manifestations of Japanese encephalitis virus infection are often acute encephalitis,and etiological diagnosis is a common diagnostic method.It is difficult to detect and isolate viruses clinically,and the types of pathogens detected are relatively limited.Metagenomics second-generation sequencing(mNGS)is a rapid and accurate molecular diagnostic method,which can improve the detection rate and types of pathogenic microorganisms.Pathogens uncommon in central nervous system infections can be detected.This paper reports a 27-year-old male patient with nausea and vomiting,high fever,disturbance of consciousness,and multiple organ failure as the main clinical manifestations.Japanese encephalitis virus was considered clinically,but etiological evidence was lacking.Japanese encephalitis virus was detected by cerebrospinal fluid mNGS,which greatly improved the accuracy of diagnosis of Japanese encephalitis virus infection.

关 键 词：脑炎 病毒性 脑炎病毒 日本 脑脊液 宏基因组学二代测序 

分 类 号：R512.32[医药卫生—内科学]