不同盐浓度处理下的紫球藻转录组测序分析  

Transcriptomic Analysis of Porphyridium purpureum Under Different Salinity Treatments

作　　者：高帆[1,2] 宋韡 汤丽群 GAO Fan;SONG Wei;TANG Liqun(School of Life Science,Shanxi University,Taiyuan 030006,China;Academic Affairs Office,Shanxi University,Taiyuan 030006,China;Shanxi Sports Vocational School,Taiyuan 030036,China)

机构地区：[1]山西大学生命科学学院,山西太原030006 [2]山西大学教务处,山西太原030006 [3]山西体育职业学院,山西太原030036

出　　处：《山西大学学报（自然科学版）》2024年第4期854-864,共11页Journal of Shanxi University(Natural Science Edition)

基　　金：山西省基础研究计划(自由探索类)(202103021224009);教育部产学合作协同育人项目(220504697124957);山西省高等学校教学改革创新项目(J20220046);山西省科技战略研究专项(202204031401051)。

摘　　要：转录组测序是挖掘调控微藻独特生物学功能及代谢通路关键基因的重要技术手段之一,但目前国内外对于不同盐浓度处理下的紫球藻(Porphyridium)转录组测序研究很少。为进一步挖掘不同浓度盐处理条件下紫球藻的功能基因,分析其参与的关键代谢通路,探究其在该藻类生长发育、生物活性物质合成及其产业化应用中的价值,本研究运用形态学、生理生化、分子生物及生物信息技术,分别对基础培养基条件(0.06 mol·L^(-1)NaCl)、中盐(1.0 mol·L^(-1)NaCl)和高盐(3.0 mol·L^(-1)NaCl)处理条件下的紫球藻(P.purpureum)进行特征及转录组测序分析。研究共获得65790条单基因(Unigenes),其中CDS(Coding Sequence)序列49653条,29.81%的Unigenes可被七大生物信息数据库注释,de novo组装了357条Contigs,其中N50长度5467 bp,GC(Guanine and Cytosine)占比58.26%。差异表达基因(Differentially Expressed Genes,DEGs)可划分为3大簇,其中高盐处理组相关基因单独聚为一簇。以基础培养基处理组为对照,中、高盐浓度处理组共筛选获得上调表达DEGs 34016条,下调表达DEGs 69086条,共有DEGs 15263条。不同比较组间共有DEGs的GO(Gene Ontology)富集程度最高的是细胞膜整体组分,KEGG(Kyoto Encyclopedia of Genes and Genomes)通路富集程度最高的是RNA转运。本研究所得紫球藻转录组测序数据及组装质量较高,筛选获得的DEGs及其功能信息可在该藻类高渗透压环境下生理机制研究、高品质微藻资源分子选育及模式微藻工业应用中提供重要的参考。As an essential technology to screen the key genes with the unique biological functions and involved in the important metabolic pathways in microalgae,transcriptomic sequencing has been widely utilized in algae research.However,nowadays,a few studies on the transcriptomic sequencing of Porphyridium under different salinity treatments have been reported around the world.In order to screen the functional genes of Porphyridium under different salt treatment conditions,analyze their related key metabolic pathways,and explore their values in the growth and development,synthesis of bioactive substances,and industrial application,the morphological,physiological and biochemical,molecular biological and bioinformatic techniques have been used to feature analysis and transcriptomic sequencing of P.purpureum under the basic medium(0.06 mol·L^(-1) NaCl),moderate salt(1.0 mol·L^(-1) NaCl) and high salt(3.0 mol·L^(-1) NaCl) treatments,respectively.A total of 65 790 unigenes were obtained,including 49 653 coding domain sequences(CDS).29.81% of them could be annotated among the seven bioinformatic databases.Totals of 357 contigs could be de novo assembled with a length of 5 467 bp for N50 and a GC ratio of 58.26%.Furthermore,differential expression genes(DEGs) could be divided into three major clusters via the cluster analyzing with the high salt treatment group clustered separately in this alga.Altogether,34 016 up-regulated DEGs,69 086 down-regulated DEGs and 15 263 common DEGs were screened from the medium and high salinity treatment groups with the basic medium group as a control,respectively.The highest enriched Gene Ontology(GO) of DEGs among different comparative groups was the integral component of membrane.Meanwhile,the highest enriched Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway was RNA transport.To sum up,abundant transcriptomic sequencing data with high assembly quality of P.purpureum with different salt treatments have been obtained in this study.The DEGs and their functional annotations predicted in th

关 键 词：紫球藻 耐盐性 转录组测序 差异表达基因 

分 类 号：Q949.2[生物学—植物学]