抗阻与有氧干预对小鼠胫骨前肌凋亡基因表达的影响  

Effects of Resistance and Aerobic Intervention of Apoptosis-related Genes Expression in Tibialis Anterior Muscle of Mice

作　　者：田鹏 岳慧英 宋洁 李海鹏[3] TIAN Peng;YUE Huiying;SONG Jie;LI Haipeng(Shanxi Vocational University of Engineering Science and Technology,Taiyuan 030619,China;College of Basic Medical Sciences of Shanxi University of Chinese Medicine,Taiyuan 030619,China;Zhejiang Technology University,Hangzhou 310000,China)

机构地区：[1]山西工程科技职业大学,山西太原030619 [2]山西中医药大学,山西太原030619 [3]浙江工业大学,浙江杭州310000

出　　处：《山西大学学报（自然科学版）》2024年第4期865-873,共9页Journal of Shanxi University(Natural Science Edition)

基　　金：山西省基础研究计划(自由探索类)面上项目(202203021212345);山西省教育规划课题(TY-230016);山西省哲学社会科学项目(2023YJ148)。

摘　　要：为探讨不同运动方式干预不同月龄SAMP8小鼠(senescence accelerated mouse prone/8)胫骨前肌细胞凋亡的效果和影响,本研究选用青年期(3月龄)和老年期(6月龄)SAMP8小鼠作为实验动物,制定抗阻(负重爬梯)和有氧(跑台)两种运动模型,分析6周运动干预后SAMP8小鼠胫骨前肌内源性细胞凋亡信号通路中关键基因及其调节基因的变化。研究结果表明,和青年组相比,老年组小鼠胫骨前肌细胞中凋亡信号Cyt C、Apaf-1和Caspase-3基因的表达量均显著上调;与对照相比,抗阻运动干预的青年组小鼠Cyt C和Caspase-9 mRNA表达量显著上调,老年组Apaf-1、Caspase-9和Caspase-3 mRNA表达量显著下调(P<0.05);有氧运动干预的青年组小鼠Apaf-1和Caspase-9相对表达量均显著上调,老年组Cyt C和Caspase-3表达量显著下调(P<0.05)。对于凋亡相关调节基因,抗阻和有氧运动干预后青年组Bcl-2基因表达量较对照显著上调,而两种运动干预下老年组Bcl-2、Bax和HSP70基因表达量均显著下调(P<0.05)。综合分析,抗阻和有氧干预均能够弱化胫骨前肌的凋亡信号,抗阻运动通过Apaf-1和Caspase-9的下调来降低老年组胫骨前肌中Caspase-3的表达,有氧运动对胫骨前肌中Caspase-9的下调作用则主要通过Cyt C释放的减少来完成;两种运动干预对青年组中调节基因的影响倾向于抑制细胞凋亡,主要通过Bcl-2上调来完成,而运动干预后下调了老年组骨骼肌中凋亡基因的表达,可能与干预时间较短有关。The present study is designed to determine the effects of resistance exercise and aerobic exercise on apoptotic signaling pathway in tibialis anterior muscles of different aged mice.Using young(3 months old) and older(6 months old) male senescence accelerated mouse prone/8(SAMP8) mice as the research animals,the resistance(ladder climbing) and aerobic(treadmill running)exercise models were established.The changes of key genes and their regulatory genes in endogenous apoptosis signaling pathway of tibialis anterior muscle in SAMP8 mice after 6-week exercise intervention were analyzed.Compared with the young group,the expression levels of Cyt C,Apaf-1 and Caspase-3 genes were significantly up-regulated in the old group,the Cyt C and Caspase-9were up-regulated in young resistance training group,whereas Apaf-1,Caspase-9 and Caspase-3 showed significantly down-regulation in old resistance training group(P<0.05).After the aerobic exercise,the Apaf-1 and Caspase-9 mRNA expression increased significantly in young mice,and expression of Cyt C and Caspase-3 decreased significantly in old groups(P<0.05).For apoptosis-related regulatory factors,Bcl-2 gene expression in the young group was significantly up-regulated compared with the control group after resistance and aerobic exercise intervention,while Bcl-2,Bax and HSP70 gene expression in the old group were significantly downregulated under the two forms of training intervention(P<0.05).Both resistance exercise and aerobic exercise can weaken the apoptotic signal in aging tibialis anterior muscles.Resistance exercise can reduce the expression of Caspase-3 in the tibialis anterior muscles of the aged group through the down-regulation of Apaf-1 and Caspase-9,whereas the down-regulation effect on Caspase-3through aerobic training in the tibialis anterior muscle was mainly achieved by the decrease of Cyt C expression.Overall,the effects of the two forms of exercise interventions on the regulatory factors in the young groups tended to inhibit apoptosis,mainly through the up-reg

关 键 词：运动干预 细胞凋亡 调控基因 

分 类 号：G804.7[文化科学—运动人体科学]