灯盏乙素对激活的小胶质细胞中PI3K表达和磷酸化水平的影响  

The effects of scutellarin on PI3K expression and phosphorylation level in activated microglia

作　　者：杨丽芳 段兆达 杨毓甲 徐冬垚 吴春云 杨力 YANG Lifang;DUAN Zhaoda;YANG Yujia;XU Dongyao;WU Chunyun;YANG Li(Department of Human Anatomy,Histology and Embryology,School of Basic medicine,Kunming Medical University,Kunming 650500,China)

机构地区：[1]昆明医科大学基础医学院人体解剖与组织胚胎学系,昆明650500

出　　处：《神经解剖学杂志》2024年第3期320-326,共7页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81960223);云南省科技厅昆医联合专项(202301AY070001-163)。

摘　　要：目的:探究灯盏乙素对激活的小胶质细胞磷脂酰肌醇3-激酶(PI3K)和磷酸化PI3K(p-PI3K)水平的影响。方法:用大鼠大脑中动脉闭塞(MCAO)法制备脑缺血模型,大鼠随机分成假手术组(Sham)、脑缺血组(MCAO)、脑缺血+灯盏乙素组(MCAO+S)。利用糖氧剥夺(OGD)法制备BV2小胶质细胞缺血缺氧损伤模型,随机分成对照组(Control)、OGD组、OGD+灯盏乙素组(OGD+S)。运用免疫荧光双标染色和Western Blot法检测不同组中小胶质细胞PI3K及PI3K磷酸化水平的变化。结果:体内和体外免疫荧光及Western Blot结果均显示,激活的小胶质细胞中PI3K磷酸化水平明显增加(P<0.05),灯盏乙素干预后PI3K磷酸化水平进一步升高(P<0.05);PI3K在各组间无明显变化(P>0.05)。结论:灯盏乙素可能通过上调激活的小胶质细胞中PI3K的磷酸化水平,对脑缺血发挥积极的治疗作用。Objective:To explore the impact of scutellarin on the levels of phosphatidylinositol 3-kinase(PI3K)and phosphorylated PI3K(p-PI3K)in activated microglia.Methods:A rat cerebral ischemia model was made by middle cerebral artery occlusion(MCAO)method.SD rats were randomly divided into three groups:sham operation group(Sham),cerebral ischemia group(MCAO),and cerebral ischemia with scutellarin group(MCAO+S).A model of ischemia-hypoxia injury of BV2 microglia was established by glucose-oxygen deprivation(OGD)and randomly assigned as control group(Control),OGD group and OGD+scutellarin group(OGD+S).Changes in PI3K and p-PI3K expression in microglia were assessed using double immunofluorescence staining and Western Blot.Results:Immunofluorescence staining and Western Blot analyses revealed a significant increase in p-PI3K levels in activated microglia both in vivo and in vitro(P<0.05).Furthermore,treatment with scutellarin led to a further elevation in p-PI3K(P<0.05).However,there were no significant alterations observed in PI3K expression among the groups(P>0.05).Conclusion:Scutellarin may play a positive role in the treatment of cerebral ischemia by up-regulating the phosphorylation level of PI3K in activated microglia.

关 键 词：脑缺血 灯盏乙素 小胶质细胞 磷脂酰肌醇3-激酶 大鼠 

分 类 号：R285[医药卫生—中药学]