1700 nm波段超快光纤光源开发及其在多光子成像中的应用  

Development of an Ultrafast Fiber Laser in 1700-nm Waveband and Its Application in Multiphoton Microscopy

作　　者：仝申 钟金成 陈新林 邱娉[1] 王科[1] Tong Shen;Zhong Jincheng;Chen Xinlin;Qiu Ping;Wang Ke(Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,College of Physics and Optoelectronic Engineering,Shenzhen University,Shenzhen 518060,Guangdong,China;School of Medical Information and Engineering,Xuzhou Medical University,Xuzhou 221004,Jiangsu,China;Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou 325001,Zhejiang,China)

机构地区：[1]深圳大学物理与光电工程学院光电子器件与系统教育部/广东省光电子器件与系统重点实验室,广东深圳518060 [2]徐州医科大学医学信息与工程学院,江苏徐州221004 [3]中国科学院大学温州研究院,浙江温州325001

出　　处：《激光与光电子学进展》2024年第12期1-18,共18页Laser & Optoelectronics Progress

基　　金：国家自然科学基金(62075135,61975126);深圳市光子学与生物光子学重点实验室(ZDSYS20210623092006020)。

摘　　要：多光子成像在生物医学特别是脑科学研究中应用广泛,为深层生物组织结构及动力学研究提供了非侵入性和非破坏性的成像手段。生物组织的吸收和强散射特性限制了成像深度。近年来,得益于原理及技术上的进步,多光子成像在活体生物中的成像深度显著提高。其中,1700 nm波段激发的多光子成像显著降低组织吸收及散射,与其他激发波段相比获得了目前最大的成像深度。本文首先介绍多光子显微成像原理,并利用孤子自频移效应构建1700 nm波段飞秒脉冲光源。在此基础上,介绍1700 nm波段多光子成像在活体小鼠脑部、皮肤结构,以及血流动力学成像中的应用。最后总结分析1700 nm波段激发多光子成像目前遇到的挑战和未来发展方向。Multiphoton imaging is extensively used in biomedical research,especially in brain science,providing noninvasive and nondestructive imaging methods for investigating the structure and dynamics of deep biological tissues.However,the absorption and strong scattering characteristics of these biological tissues limit the depth of imaging.Recently,owing to advances in principles and technology,remarkable improvements have been realized in multiphoton imaging with respect to imaging depth in living organisms.Among them,multiphoton imaging obtained by exciting the 1700 nm wavelength band considerably reduces tissue absorption and scattering,achieving the current maximum imaging depth compared with other excitation bands.This article introduces the imaging principles of multiphoton microscopy and utilizes the soliton self-frequency shift effect to construct a femtosecond pulse light source with a 1700 nm band.Furthermore,the application of multiphoton imaging in live mouse brain,skin structure,and hemodynamic imaging is discussed.Finally,the current challenges and future development directions of 1700 nm excitation multiphoton imaging are analyzed and summarized.

关 键 词：多光子显微成像 孤子自频移 1700 nm波段 脑成像 皮肤成像 

分 类 号：O437[机械工程—光学工程] Q63[理学—光学]