一种高灵敏度探针式术中甲状旁腺识别装置  

Highly Sensitive Intraoperative Parathyroid Identification Device with Fiber-Optic Probe

作　　者：陈亮[1] 陈曦 李健聪[2] 王越桥 徐荣青 Chen Liang;Chen Xi;Li Jiancong;Wang Yueqiao;Xu Rongqing(College of electronic and optical engineering,Nanjing University of Posts and Telecommunications,Nanjing 210003,Jiangsu,China;Beneeon(Jiangsu)Medical Technology Co.,Ltd,Taizhou 225300,Jiangsu,China;School of electronic and optical Engineering,Nanjing University of Science and Technology,Nanjing 210094,Jiangsu,China)

机构地区：[1]南京邮电大学电子与光学工程学院,江苏南京210003 [2]江苏百宁盈创医疗科技有限公司,江苏泰州225300 [3]南京理工大学电子工程与光电技术学院,江苏南京210094

出　　处：《激光与光电子学进展》2024年第12期240-247,共8页Laser & Optoelectronics Progress

基　　金：江苏省科技专项资金(BE2023762);泰州市科技支撑计划(TG202220)。

摘　　要：基于甲状旁腺的近红外自体荧光特性,开发出一款高灵敏激光诱导荧光检测(LIF)技术的术中甲状旁腺快速识别装置。通过一种新型近红外传感单元组成的荧光采集模块和改进型荧光检测光路设计,实现了光纤探针式术中甲状旁腺快速、无创识别装置。通过实验验证吲哚菁绿(ICG)溶液与甲状旁腺的荧光光谱具有极大相似性,通过吲哚菁绿(ICG)溶液模拟甲状旁腺自体荧光特性的实验,测得该装置在低至0.05 nmol/L ICG的实验条件下,荧光强度仍然与ICG浓度成正比,荧光强度和ICG浓度的线性相关系数高达0.999,与理论高度吻合。使用该装置在广州市第一人民医院进行临床实验,实验结果显示该装置能够成功激发出甲状旁腺自体荧光,荧光强度约为其他组织的5倍,装置检测结果与临床病理切片结果高度吻合,证明本文研制的装置能够明显区分甲状旁腺和其他组织。临床专家给出结论,该装置能够快速、无创、精准识别甲状旁腺组织,具有巨大的临床价值。Based on the near-infrared autofluorescence characteristics of a parathyroid gland,a rapid intraoperative parathyroid identification device using highly sensitive laser-induced fluorescence(LIF)technology was developed.This paper describes the development of a fiber-optic probe-based non-invasive identification device for intraoperative parathyroid recognition.The device used a novel near-infrared sensing unit incorporated into a fluorescence collection module.By performing fluorescence experiments using indocyanine green(ICG)solution as a simulation of parathyroid autofluorescence,it was observed that the device exhibited a linear relationship between fluorescence intensity and ICG concentration,even at a low concentration of 0.05 nmol/L.The correlation coefficient between fluorescence intensity and ICG concentration was 0.999,indicating a strong correlation that closely matched the theoretical expectations.The device was tested in clinical experiments at the First People’s Hospital in Guangzhou City.The experimental results demonstrated that the device successfully elicited autofluorescence from parathyroid glands.The fluorescence intensity of the parathyroid glands was approximately five times higher than those of other tissues,allowing for clear differentiation between the parathyroid glands and other tissues.Clinical experts concluded that the device can rapidly,non-invasively,and accurately identify parathyroid tissue,providing significant clinical value.

关 键 词：甲状旁腺 激光诱导荧光检测技术 自体荧光 吲哚菁绿 光纤探针 

分 类 号：TN29[电子电信—物理电子学]