四个品种红三叶种带真菌多样性研究  

Diversity of seed-borne fungi on four Trifolium pratense cultivars

作　　者：郑荣春 南志标[1] 段廷玉[1] ZHENG Rong-chun;NAN Zhi-biao;DUAN Ting-yu(State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems,College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China)

机构地区：[1]兰州大学草种创新与草地农业生态系统全国重点实验室,兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《草业学报》2024年第8期170-180,共11页Acta Prataculturae Sinica

基　　金：国家绿肥技术产业体系(CARS-22)资助。

摘　　要：红三叶是一种重要的豆科牧草及绿肥作物,种带真菌会严重影响其种子品质,是限制生产的主要因素之一。本研究以红三叶4个品种为对象,通过室内分离及高通量测序,明确不同红三叶品种种带真菌的种类及多样性。自4个供试品种分离鉴定出种带真菌15属26种,其中岷山红三叶9属14种,瑞德红三叶5属7种,VNS红三叶9属9种,红龙红三叶7属11种。高通量测序法从红三叶4个品种种子样品中共鉴定出30属87种。属水平主要为枝孢菌属、链格孢属、青霉属、曲霉属、匍柄霉属、掷孢酵母属、Filobasidium和Udeniomyces等属。其中匍柄霉属、链格孢属、枝孢菌属、枝顶孢属、葡萄孢属、镰孢菌属及白粉菌属的数个种为潜在致病菌。α和β多样性分析表明品种间真菌群落组成及丰度差异显著,岷山的种带真菌多样性高于其他3个品种。Red clover(Trifolium pratense)is an important leguminous forage and green manure crop.However,seed-borne fungi severely affect the seed quality of red clover,and are among the major limiting factors for red clover production.In this study,four varieties of red clover were selected to investigate the species and diversity of seedborne fungi using indoor isolation and high-throughput sequencing methods.A total of 26 species from 15 genera of seedborne fungi were identified from the four tested varieties,including 14 species from nine genera in T.pratense(cv.Minshan),seven species from five genera in T.pratense(cv.Ruide),nine species from nine genera in T.pratense(cv.VNS),and 11 species from seven genera in T.pratense(cv.Honglong).High-throughput sequencing of fungi from the seed samples of the four red clover varieties identified a total of 87 species from 30 genera,including Cladosporium,Alternaria,Penicillium,Aspergillus,Stemphylium,Sporobolomyces,Filobasidium,Udeniomyces,and other genera.Among them,several species of the genera Stemphylium,Alternaria,Cladosporium,Acremonium,Botrytis,Fusarium,and Erysiphe were identified as potential pathogens.Alpha and beta diversity analyses revealed significant differences in fungal community composition and abundance among the red clover varieties,with T.pratense(cv.Minshan)exhibiting higher diversity of seed-borne fungi compared with that of the other three red clover varieties.

关 键 词：红三叶 种带真菌 高通量测序 多样性 

分 类 号：S541.9[农业科学—作物学]