血清微RNA-130b-3p、果蝇母系DPP同源物4对多囊卵巢综合征不孕病人人工授精妊娠结局的预测价值  

Predictive value of serum miR-130b-3p and SMAD4 for pregnancy outcomes after artificial insemination in infertile patients with polycystic ovary syndrome

作　　者：乔婷 孟晓楠 马晓娟 QIAO Ting;MENG Xiaonan;MA Xiaojuan(Department of Reproductive Genetics,Pingdingshan Maternal and Child Health Hospital,Pingdingshan,He'nan 467000,China)

机构地区：[1]平顶山市妇幼保健院生殖遗传科,河南平顶山467000

出　　处：《安徽医药》2024年第9期1842-1846,共5页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探究血清微RNA(miR)-130b-3p、果蝇母系DPP同源物4(SMAD4)对多囊卵巢综合征(PCOS)不孕病人人工授精妊娠结局的预测价值。方法选取2018年1月至2022年1月在平顶山市妇幼保健院就诊的PCOS不孕病人172例为PCOS组,另取同期体检的180例月经周期正常且已婚已育女性作为对照组。实时荧光定量PCR法检测血清中miR-130b-3p的水平;用酶联免疫吸附测定(ELISA)法检测血清SMAD4水平。判断PCOS病人人工授精结局并分为临床妊娠组(n=146)和未临床妊娠组(n=26)。Pearson法对PCOS病人血清SMAD4、miR-130b-3p、抗苗勒氏管激素(AMH)的相关性进行分析;logistic回归分析影响PCOS不孕病人人工授精后未临床妊娠的危险因素,采用受试者操作特征曲线(ROC曲线)评估miR-130b-3p、SMAD4水平预测PCOS病人人工授精未临床妊娠的价值。结果与对照组相比,PCOS组血清miR-130b-3p(1.02±0.24比2.16±0.34)水平较高(t=36.47,P<0.05),血清SMAD4[(71.15±11.62)ng/L比(36.05±6.37)ng/L]水平较低(t=34.92,P<0.05),与临床妊娠组相比,未临床妊娠组血清miR-130b-3p(2.04±0.31比2.85±0.51)水平较高(P<0.05),优势卵泡数≥2个比例(78.08%比50.00%)及血清AMH[(8.27±1.85)pg/L比(6.16±1.01)pg/L]、SMAD4[(38.27±6.99)ng/L比(23.60±2.91)ng/L]水平较低(P<0.05)。Pearson分析显示,PCOS病人血清中SMAD4与miR-130b-3p呈负相关(r=−0.56,P<0.05),PCOS病人血清中miR-130b-3p与AMH呈负相关(r=−0.46,P<0.05),血清中SMAD4与AMH呈正相关(r=0.46,P<0.05)。logistic分析显示,miR-130b-3p水平升高是PCOS不孕病人人工授精后未临床妊娠的危险因素(P<0.05),AMH和SMAD4水平降低是PCOS不孕病人人工授精后未临床妊娠的保护因素(P<0.05)。ROC曲线分析显示,血清miR-130b-3p、SMAD4联合预测PCOS病人人工授精未临床妊娠的曲线下面积(AUC)为0.95,其灵敏度为96.20%,特异度76.70%。结论PCOS病人血清miR-130b-3p水平升高,SMAD4水平下降,二者对PCOS病人人工授精未临床妊娠有预测�Objective To investigate the predictive value of serum microRNA(miR)-130b-3p and drosophila mothers against decap-entaplegic 4(SMAD4)in Drosophila mothers on pregnancy outcomes after artificial insemination in infertile patients with polycystic ovary syndrome(PCOS).Methods From January 2018 to January 2022,172 infertile patients with PCOS treated at the Pingdingshan Maternal and Child Health Hospital composed the PCOS group,and another 180 women of child-bearing age with normal menstrual cy-cles who underwent physical examination composed the control group during the same period.The levels of miR-130b-3p in the serum were detected by real-time fluorescence quantitative PCR,and the levels of serum SMAD4 were determined by enzyme-linked immuno-sorbent assay(ELISA).The artificial insemination outcomes of PCOS patients were evaluated,and the patients were grouped into a clin-ical pregnancy group(n=146)and a nonclinical pregnancy group(n=26).The correlation between serum SMAD4,miR-130b-3p and an-ti-Müllerian hormone(AMH)in PCOS patients was analyzed by the Pearson method.Logistic regression was performed to analyze the risk factors affecting nonclinical pregnancies after intrauterine insemination in infertile PCOS patients,and receiver operating charac-teristic(ROC)curves were used to evaluate the value of miR-130b-3p and SMAD4 levels in predicting nonclinical pregnancy after arti-ficial insemination in PCOS patients.Results Compared with those in the control group,the serum levels of miR-130b-3p(1.02±0.24 vs.2.16±0.34)in the PCOS group were greater(t=36.47,P<0.05),and the serum levels of SMAD4[(71.15±11.62)ng/L vs.(36.05±6.37)ng/L]were lower(t=34.92,P<0.05).Compared with those in the clinical pregnancy group,the serum levels of miR-130b-3p(2.04±0.31 vs.2.85±0.51)were greater,while the proportions of dominant follicles≥2(78.08%vs.50.00%),serum AMH[(8.27±1.85)pg/L vs.(6.16±1.01)pg/L]and SMAD4[(38.27±6.99)ng/L vs.(23.60±2.91)ng/L]were lower(P<0.05)in the nonclinical pregnancy group.Pear-son analysis reveale

关 键 词：多囊卵巢综合征 微RNA-130b-3p 果蝇母系DPP同源物4 不孕 人工授精 

分 类 号：R714.8[医药卫生—妇产科学]