基于JAK2/STAT3信号通路研究BMSC移植对PDPN的影响  

Effect of BMSC Transplantation on PDPN Based on JAK2/STAT3 Signal Pathway

作　　者：崔志龙 许胜炜 黄飞扬 闫思羽 祝天赐 张萌[1] CUI Zhilong;XU Shengwei;HUANG Feiyang(Basic Medical College,Qiqihar Medical Collge,Heilongjiang 161006,China)

机构地区：[1]齐齐哈尔医学院基础医学院,161006 [2]齐齐哈尔医学院精神卫生学院,161006

出　　处：《医学研究杂志》2024年第7期120-124,共5页Journal of Medical Research

基　　金：黑龙江省省属本科高校基本科研业务费科学技术研究项目(2020-KYYWF-0011)。

摘　　要：目的探讨骨髓间充质干细胞(bone marrow mesenchymal stemcell,BMSC)移植对痛性糖尿病周围神经病变(painful diabetic peripheral neuropathy,PDPN)大鼠的影响。方法选取SPF级雄性GK大鼠14只,随机分为模型组和治疗组,每组各7只。另选取同周龄、同性别Wistar大鼠7只作为正常组。治疗组大鼠腹腔注射BMSC进行6周治疗。检测热痛阈;取坐骨神经,采用苏木精-伊红(hematoxylin-eosin,HE)染色、Masson染色观察坐骨神经病理学形态改变;采用Western blot法检测JAK2/STAT3信号通路相关蛋白P-JAK2、P-STAT3的表达。结果经BMSC治疗后,大鼠热痛阈值有所上升;坐骨神经损伤被修复,炎性细胞浸润缓解及脱髓腔现象改善;通路相关蛋白p-JAK2、P-STAT3表达下降。结论BMSC移植通过抑制JAK2/STAT3信号通路能减轻炎性反应,修复坐骨神经,缓解疼痛。Objective To investigate the effect of bone marrow mesenchymal stem cells(BMSC)transplantation on rats with painful diabetes peripheral neuropathy(PDPN).Methods Fourteen SPF grade male GK rats were randomly divided into model group and treat-ment group,with 7 rats in each group.Seven Wistar rats of the same-sex and same-age were used as the normal group.The treatment group received intraperitoneal injection of BMSC for 6 weeks of treatment.Detection of thermal pain threshold;take the sciatic nerve and observe the pathological morphological changes of the sciatic nerve using hematoxylin-eosin(HE)staining and Masson staining;Western blot was used to detect the expression of JAK2/STAT3 signaling pathway related proteins p-JAK2 and p-STAT3.Results Ater treat-ment with BMSC,the thermal pain threshold of rats increased slightly;the sciatic nerve injury was repaired,the infiltration of inflammato-ry cells was alleviated,and the phenomenon of demyelination was improved;the expression of pathway related proteins p-JAK2 and p-STAT3 decreased.Conclusion BMSC transplantation can alleviate inflammatory response,repair sciatic nerve,and alleviate pain by in-hibiting the JAK2/STAT3 signaling pathway.

关 键 词：骨髓间充质干细胞 JAK2/STAT3信号通路 炎症 痛性糖尿病神经病变 

分 类 号：R587[医药卫生—内分泌]