儿泻康贴膜对功能性腹痛幼龄大鼠药效作用及机制研究  

Efficacy and mechanism of Erxiekang Plaster on functional abdominal pain juvenile rats

作　　者：宋紫腾 王甜[5] 罗春 黄泽森 朱加华 侯淇允 余彩霞 张嘉慧 韩彦琪[1,2,3,4] 许浚 赵专友[1,2,3,4] 刘昌孝 张铁军[1,2,3,4] SONG Ziteng;WANG Tian;LUO Chun;HUANG Zesen;ZHU Jiahua;HOU Qiyun;YU Caixia;ZHANG Jiahui;HAN Yanqi;XU Jun;ZHAO Zhuanyou;LIU Changxiao;ZHANG Tiejun(National Key Laboratory of Druggability Evaluation and Systematic Translational Medicine,Tianjin Institute of Pharmaceutical Research,Tianjin 300462,China;National&Local United Engineering Laboratory of Modern Preparation and Quality Control Technology of Traditional Chinese Medicine,Tianjin Institute of Pharmaceutical Research,Tianjin 300462,China;Tianjin Key Laboratory of Quality Marker of Traditional Chinese Medicine,Tianjin Institute of Pharmaceutical Research,Tianjin 300462,China;Heguang Chinese Medicine Technology(Tianjin)Co.,Ltd.,Tianjin 300462,China;Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Shanghai Children's Nutrition Center,Shanghai 201403,China)

机构地区：[1]天津药物研究院药物成药性评价与系统转化全国重点实验室,天津300462 [2]天津药物研究院中药现代制剂与质量控制技术国家地方联合工程实验室,天津300462 [3]天津药物研究院天津市中药质量标志物重点实验室,天津300462 [4]和光中药科技(天津)有限公司,天津300462 [5]天津中医药大学,天津301617 [6]上海儿童营养中心,上海201403

出　　处：《现代药物与临床》2024年第7期1653-1661,共9页Drugs & Clinic

基　　金：安徽省科技重大专项项目(202303a0702007)。

摘　　要：目的 研究儿泻康贴膜对功能性腹痛幼龄大鼠的影响,并探讨其疗效机制。方法 将出生1 d的SD大鼠随机分为对照组和模型组,采用母婴分离联合冰水应激的方法建立功能性腹痛大鼠内脏高敏感模型。选取造模成功的大鼠随机分为模型组、丁桂儿脐贴组,儿泻康贴膜(1/24、1/12、1/6贴)组。各组大鼠腹部贴剂给药,连续7 d,对照组、模型组腹部给予空白模拟贴。观察给药后大鼠粪便Bristol分级评分、粪便含水率及腹壁撤退反射(AWR)评分结果;采用酶联免疫吸附实验(ELISA)法检测血清和结肠组织中5-羟色胺(5-HT)、P物质(SP)、肿瘤坏死因子-α(TNF-α)的含量;采用实时荧光定量聚合酶链式反应(RT-qPCR)测定结肠组织中囊性纤维化跨膜转运调节体(CFTR)、促肾上腺皮质激素释放因子(CRF)m RNA表达水平;Western blotting法检测CFTR、CRF蛋白表达水平。结果 与模型组相比,儿泻康贴膜各剂量组大鼠粪便Bristol评分明显降低,粪便含水量显著减少,不同注水体积下的AWR评分均显著降低,血清和结肠组织中致痛致泻相关因子5-HT、SP、TNF-α的表达均得到显著抑制,结肠组织中CFTR和CRF mRNA和蛋白的相对表达量均显著降低(P<0.05、0.01、0.001)。结论 儿泻康贴膜对功能性腹痛大鼠有显著的治疗效果,其通过调节血清和结肠组织中腹痛腹泻相关因子的含量以及结肠组织中相关蛋白的表达以显著降低大鼠粪便Bristol评分和粪便含水量以及大鼠的内脏敏感性。Objective To observe effects of Erxiekang Plaster on functional abdominal pain juvenile rats,and explore its mechanism.Methods The 1-day-old SD rats were randomly divided into control group and model group.The functional abdominal pain rat models with visceral hypersensitivity were established by the method of combination of maternal separation and ice water stress.The rats with successful modeling were randomly divided into model group,Dingguier Umbilical Paste group,and Erxiekang Plaster(1/24,1/12,1/6)group.Each group of rats was given abdominal patches for 7 consecutive days,while the control group and model group were given blank simulated patches on the abdomen.Scores of Bristol classification and water content of the fecal pellets and abdominal withdrawal reflex(AWR)of rats in all the groups were observed after treatment.ELISA was used to detect the changes of 5-HT,SP and TNF-αin serum and colon tissue.The mRNA expression levels of CFTR and CRF in colonic tissues were detected by RT-PCR.The protein expression levels of both were detected by Western blotting.Results Compared with the model group,the Bristol score and fecal water content of rats significantly decreased after treatment with Erxiekang Plaster,and the AWR score significantly decreased under different water injection volumes.The expression levels of 5-HT,SP,and TNF-αin serum and colon tissue were significantly decreased.The CFTR and CRF mRNA and protein expression significantly down-regulated.Conclusion Erxiekang Plaster has a significant therapeutic effect on functional abdominal pain in rats.It can significantly reduce the Bristol score and fecal water content,as well as visceral sensitivity of rats by regulating the content of abdominal pain and diarrhea related factors in serum and colon tissue,as well as the expression of related proteins in colon tissue.

关 键 词：儿泻康贴膜 儿童功能性腹痛 5-羟色胺 P物质 肿瘤坏死因子-α 囊性纤维化跨膜转运调节体 促肾上腺皮质激素释放因子 

分 类 号：R285.5[医药卫生—中药学]