健脾止动汤对多发性抽动症模型大鼠组胺及组胺脱羧酶的影响  

Effect of Jianpi Zhidong Decoction on the Expression of Histamine and Histamine Decarboxylase in Tourette Syndrome Model Rats

作　　者：于文静 王素梅[2] 史晓伟 白雪[3] 张雯 王倩 赵颖 崔霞 YU Wenjing;WANG Sumei;SHI Xiaowei;BAI Xue;ZHANG Wen;WANG Qian;ZHAO Ying;CUI Xia(Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China;Beijing University of Chinese Medicine Dongfang Hospital,Beijing 100078,China;Beijing University of Chinese Medicine,Beijing 100105,China)

机构地区：[1]北京中医药大学第三附属医院,北京100029 [2]北京中医药大学东方医院,北京100078 [3]北京中医药大学,北京100105

出　　处：《世界中医药》2024年第15期2265-2270,共6页World Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目(82305317);北京市自然科学基金项目(7232282);北京中医药大学中青年教师项目(2017-JYB-JS-125)。

摘　　要：目的:探讨健脾止动汤对多发性抽动症(TS)模型大鼠纹状体及血清组胺(HA)、组胺脱羧酶(HDC)表达的影响。方法:将24只SD大鼠按照随机数字表法分为空白组、模型组、中药组和西药组,每组6只。除空白组外,其他组均给予250 mg/kg IDPN腹腔注射7 d构建TS模型。空白组、模型组用15 mL/kg生理盐水灌胃,中药组用16 g/kg健脾止动汤灌胃,西药组给予21 mg/kg盐酸硫必利灌胃,1次/d,连续干预6周。对比各组大鼠体质量、行为学变化。用酶联免疫吸附试验(ELISA)检测大鼠纹状体及血清HA,用反转录聚合酶链反应(RT-PCR)检测大鼠纹状体HDC mRNA表达,用蛋白质印迹法和免疫组织化学法检测大鼠纹状体HDC蛋白表达。结果:模型组、健脾止动汤组、西药组体质量比较差异无统计学意义(P>0.05);干预6周,与模型组比较,健脾止动汤组、西药组体质量均低(P<0.05)。模型组、健脾止动汤组、西药组行为学评分比较差异无统计学意义(P>0.05);干预6周,与模型组比较,健脾止动汤组、西药组行为学评分均低(P<0.01)。与空白组比较,模型组大鼠血清HA水平低(P<0.05);与模型组比较,健脾止动汤组大鼠血清HA水平高(P<0.05),西药组血清HA水平高,但差异无统计学意义(P>0.05)。与空白组比较,模型组大鼠纹状体HA水平低(P<0.05);与模型组比较,健脾止动汤组及西药组大鼠纹状体HA水平高(P<0.01)。与空白组比较,模型组纹状体HDC mRNA表达量低,但差异无统计学意义(P>0.05);与模型组比较,健脾止动汤组及西药组大鼠纹状体HDC mRNA表达量高,但差异无统计学意义(P>0.05)。蛋白质印迹法检测结果:与空白组比较,模型组大鼠纹状体HDC蛋白表达低(P<0.05);与模型组比较,健脾止动汤组纹状体HDC蛋白表达高(P<0.05),西药组纹状体HDC蛋白表达高,但差异无统计学意义(P>0.05)。免疫组织化学法检测结果:与空白组比较,模型组纹状体组胺脱羧酶IOD值低(P<0.05);�Objective:To investigate the effect of Jianpi Zhidong Decoction on the histamine(HA)and histidine decarboxylase(HDC)expression in the striatum and serum in a Tourette syndrome(TS)rat model.Methods:Twenty-four SD rats were randomly assigned to blank,model,Chinese medicine,and western medicine groups according to a random number table,with six rats in each group.Except for the blank group,all other groups were given IDPN at 250 mg/kg via intraperitoneal injection for 7 days to establish the TS model.The blank and model groups were administered with saline at 15 mL/kg,the Chinese medicine group with Jianpi Zhidong Decoction at 16 g/kg,and the western medicine group with sulpiride at 21 mg/kg,once daily,for 6 weeks.Body weight and behavioral changes of all groups were compared.Enzyme-linked immunosorbent assay(ELISA)was used to measure HA levels in the striatum and serum.Reverse transcription polymerase chain reaction(RT-PCR)was used to detect HDC mRNA expression in the striatum.Western blot and immunohistochemistry were used to assess HDC protein expression in the striatum.Results:There were no significant differences in body weight among the model,Jianpi Zhidong Decoction,and western medicine groups(P>0.05).After 6 weeks of intervention,both the Jianpi Zhidong Decoction and western medicine groups had lower body weight than the model group(P<0.05).Behavioral scores showed no significant differences between the model,Jianpi Zhidong Decoction,and western medicine groups(P>0.05).After 6 weeks of intervention,the Jianpi Zhidong Decoction and western medicine groups had lower scores than the model group(P<0.01).Compared with the blank group,the model group showed lower serum HA levels(P<0.05).However,serum HA levels were higher in the Jianpi Zhidong Decoction group than in the model group(P<0.05),while the western medicine group showed a non-significant increase(P>0.05).Striatal HA levels were lower in the model group than in the blank group(P<0.05),but higher in the Jianpi Zhidong Decoction and western medicine groups

关 键 词：健脾止动汤 抑木扶土 大鼠 多发性抽动症 血清 纹状体 组胺脱羧酶 组胺 

分 类 号：R242[医药卫生—中医临床基础] R745.1[医药卫生—中医学]