土大黄提取物对银屑病小鼠P物质及其神经肽受体-1表达的影响  

Effect of Rumex obtusifolius L.Extract on Substance P and Neurokinin-1 Receptor Expression in Psoriasis Mice

作　　者：热比姑丽·伊斯拉木 优力都孜·买买提 玉素甫江·艾力 开丽比努尔·阿布来提 巴合沙拉·马乃甫 RABIGUL·Islam;YULTUZ·Mamat;YUSUPJAN Al;KALBINUR·Ablat;BAHSARA·Manap(Institute of Xinjiang Traditional Uyghur Medicine,Urumqi 830000,China;Laboratory of Traditional Uyghur Medicine Prescription of Xinjiang,Urumqi 830000,China;Collage of Traditional Uyghur Medicine,Xinjiang Medical University,Urumqi 830000,China)

机构地区：[1]新疆维吾尔自治区维吾尔医药研究所,乌鲁木齐830000 [2]新疆维吾尔医方剂学实验室,乌鲁木齐830000 [3]新疆医科大学维吾尔医学院,乌鲁木齐市830000

出　　处：《世界中医药》2024年第15期2271-2278,共8页World Chinese Medicine

基　　金：国家自然科学基金项目(81060314);新疆维吾尔自治区自然科学基金面上项目(2021D01A158);新疆维吾尔自治区卫生健康委青年科技人才专项(2015Y40);新疆维吾尔自治区公益性科研院所专项(KY2015138,KY2015139);新疆维吾尔自治区重点实验室维吾尔医方剂学实验室开放课题(XJYS1104-2012-04)。

摘　　要：目的:经土大黄干预,探讨P物质(SP)及其神经激肽-1受体(NK-1R)在银屑病皮肤组织中的表达及意义。方法:收集对照组,银屑病模型组,甲氨蝶呤片组(1.3 mg/kg),阿瑞匹坦胶囊组(5 mg/kg),土大黄组(1、2、4 g/kg)小鼠皮肤、血液标本,经苏木精-伊红(HE)染色,观察银屑病小鼠皮肤组织病理学变化;采用实时荧光定量聚合酶链反应(RT-qPCR)技术,检测银屑病小鼠皮肤SP、NK-1R mRNA表达;采用酶联免疫吸附测定、蛋白免疫印迹技术,检测银屑病小鼠血液SP、皮肤、NK-1R蛋白表达;随后分析SP、NK-1R表达水平与银屑病的关系。结果:与银屑病模型组比较,所有药物干预组均能改善银屑病小鼠皮肤组织病理学变化;同时,小鼠皮肤SP、NK-1RmRNA表达,血清SP、皮肤NK-1R蛋白表达均降低(P<0.05,P<0.01)。与阳性对照药甲氨蝶呤片组、阿瑞匹坦胶囊组比较,1 g/kg大黄组小鼠皮肤组织NK-1R蛋白表达升高(P<0.05)。与土大黄组(1 g/kg)比较,土大黄组(2、4 g/kg)小鼠皮肤组织NK-1R蛋白表达均降低,其中土大黄组(2 g/kg)(P>0.05)、土大黄组(4 g/kg)(P<0.05)。结论:土大黄可能通过降低焦虑相关神经递质SP及其NK-1R受体基因、蛋白表达,从而改善小鼠银屑病样皮损,其作用不亚于阳性对照药甲氨蝶呤片、阿瑞匹坦胶囊。Objective:To investigate the expression and significance of substance P(SP)and its neurokinin-1 receptor(NK-1R)in psoriatic skin tissue after intervention with Rumex obtusifolius L.Methods:Skin and blood samples were collected from control mice,psoriasis model mice,and mice treated with methotrexate tablets(1.3 mg/kg),aprepitant capsules(5 mg/kg),and R.obtusifolius at doses of 1,2,and 4 g/kg.Hematoxylin and eosin(HE)staining was used to observe the pathological changes in psoriatic skin tissues.Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)was employed to detect the expression of SP and NK-1R mRNA in psoriatic skin.Enzyme-linked immunosorbent assay(ELISA)and Western blot were used to measure the expression of SP in serum and NK-1R protein in the skin.Subsequently,the relationship between the expression levels of SP,NK-1R,and psoriasis was analyzed.Results:Compared to the psoriasis model group,all drug intervention groups showed improvements in the pathological changes of psoriatic skin tissue and reduced expression of SP and NK-1R mRNA in the skin,and SP in serum,as well as NK-1R protein in the skin(P<0.05,P<0.01).Compared to the positive control drugs methotrexate and aprepitant,the R.obtusifolius group at 1 g/kg showed increased expression of NK-1R protein in the skin tissue(P<0.05).Compared to the results in the R.obtusifolius group at 1 g/kg,the expression of NK-1R protein in the skin tissue was decreased in the R.obtusifolius groups at 2 g/kg and 4 g/kg,with the 2 g/kg group showing a non-significant decrease(P>0.05)and the 4 g/kg group showing a significant decrease(P<0.05).Conclusion:R.obtusifolius may improve psoriasis-like skin lesions in mice by reducing the expression of the anxiety-related neurotransmitter SP and its receptor NK-1R at both the gene and protein levels.Its effectiveness is comparable to the positive control drugs methotrexate and aprepitant.

关 键 词：土大黄 银屑病 小鼠 苏木精-伊红染色 实时荧光定量聚合酶链反应 酶联免疫吸附测定 蛋白免疫印迹 P物质 神经肽-1受体 

分 类 号：R282[医药卫生—中药学] R965.1[医药卫生—中医学]