圆叶青藤内生真菌Phlebiopsis crassa次生代谢产物研究  

Study on the secondary metabolites of endophytic fungus Phlebiopsis crassa from Illigera orbiculata C.Y.Wu

作　　者：赵冕 唐蓉蓉 冯子迈 刘玉衡 王意浓 蔡乐[2] 贾庆忠[3] ZHAO Mian;TANG Rong-rong;FENG Zi-mai;LIU Yu-heng;WANG Yi-nong;CAI Le;JIA Qing-zhong(Experimental Center for Teaching,Hebei Medical University,Shijazhuang 050017,China;School of Chemical Science and Technology,Yunnan University,Kunming 650091,China;College of Pharmacy,Hebei Medical University,Shijiazhuang 050017,China)

机构地区：[1]河北医科大学教学实验中心,河北石家庄050017 [2]云南大学化学科学与工程学院,云南昆明650091 [3]河北医科大学药学院,河北石家庄050017

出　　处：《化学研究与应用》2024年第8期1915-1921,共7页Chemical Research and Application

基　　金：云南省科技厅-云南大学“双一流”建设联合专项-重大项目(202201BF070001-014)资助;河北省重点研发计划项目(21372601D)资助;河北省自然科学基金项目(B2022206008)资助;河北医科大学国家级大学生创新性实验计划项目(USIP2023017)资助。

摘　　要：本文对分离自圆叶青藤(Illigera orbiculata C.Y.Wu)的一株内生真菌Phlebiopsis crassa MYYJ-5-1的次生代谢产物进行了研究,从其土豆固体发酵物中分离得到9个化合物,分别鉴定为:benzophenone(1)、p-hydroxybenzoic acid(2)、p-hydroxybenzaldehyde(3)、indole-3-carboxaldehyde(4)、indole-3-carboxylic acid(5)、thymidine(6)、uracil(7)、thymine(8)、mono-methyl succinate(9)。同时采用二倍稀释法对化合物进行体外抗植物病原菌活性测定,结果显示:化合物1和3对黄瓜萎蔫球菌具有中等抑制活性,MIC值均为32μg·mL^(-1);化合物5对人参链格孢菌具有中等抑制活性,MIC值为32μg·mL^(-1);化合物6对黑曲霉菌具有显著抑制活性,活性等同于阳性对照制霉素,MIC值均为16μg·mL^(-1)。The secondary metabolites of Phlebiopsis crassa MYYJ-5-1,an endophytic fungus derived from Illigera orbiculata C.Y.Wu were studied.Nine secondary metabolites were isolated from the potato solid fermentation of this fungus,and identified as:benzophenone(1),p-hydroxybenzoic acid(2),p-hydroxybenzaldehyde(3),indole-3-carboxaldehyde(4),indole-3-carboxylic acid(5),thymidine(6),uracil(7),thymine(8),mono-methyl succinate(9).The anti-plant pathogenic fungi activities of the isolated compounds were determined by the double dilution method.The results showed that compounds 1 and 3 possessed moderate inhibitory activity against Plectosphaerella cucumerina,and the MIC values of both compounds were 32μg·mL^(-1).Compound 5 showed moderate inhibitory activity against Alternaria panax with MIC value of 32μg·mL^(-1).Compound 6 exhibited significant inhibitory activity against Aspergillus niger,and the activity was equivalent to that of positive control nystatin,with MIC value of 16μg·mL^(-1).

关 键 词：圆叶青藤 Phlebiopsis crassa 次生代谢产物 抗植物病原菌活性 

分 类 号：O629[理学—有机化学]