磁分散固相萃取-高效液相色谱法检测人体尿样中天麻素  

Determination of gastrodin in urine by magnetic dispersion solid phase extraction-high performance liquid chromatography

作　　者：陈晓兵[1,2] 李慧文 沙鸥 戴欣成 吴赟帆 徐梓舜 王滋文 CHEN Xiao-bing;LI Hui-wen;SHA Ou;DAI Xin-cheng;WU Yun-fan;XU Zi-shun;WANG Zi-wen(Lianyungang First People's Hospital,Lianyungang 222061,China;School of Pharmacy,Jiangsu Ocean University,Lianyungang 222005,China;School of Environmental and Chemical Engineering,Jiangsu Ocean University,Lianyungang 222005,China)

机构地区：[1]连云港市第一人民医院,江苏连云港222061 [2]江苏海洋大学药学院,江苏连云港222005 [3]江苏海洋大学环境与化学工程学院,江苏连云港222005

出　　处：《化学研究与应用》2024年第8期1929-1934,共6页Chemical Research and Application

基　　金：江苏省大型科学仪器开放共享自主研究课题(202239)资助;江苏省海洋资源开发研究院开放基金课题(JSIMR202205JSIMR202206、JSIMR202207)资助;研究生科研与实践创新计划项目(KYCX2022-33)资助。

摘　　要：建立了一种磁分散固相萃取技术结合高效液相色谱法,用于快速检测人体尿液样本中的天麻素。采用化学共沉淀法自组装制备磁性活性炭/大孔树脂三元复合材料(Fe_3O_(4)/AC/MR),并将该材料用作吸附剂,实现尿液样本中天麻素的分离富集。在最佳实验条件下,天麻素浓度与峰面积间呈良好线性关系,线性范围为3.0×10^(-2)~10.0μg·mL^(-1),线性相关系数为0.9994,检出限为5.4μg·L^(-1)。以模拟人体尿液作为实际样品进行加标回收验证,加标回收率为94.87%~106.3%,相对标准偏差(RSD)在2.8%-4.2%之间。本研究建立的方法简便、快速、有效,为生物样本中天麻素的分离提纯提供了新方法。To establish a magnetic dispersion solid-phase extraction technology combined with high-performance liquid chromatography for the rapid detection of gastrodin in human urine samples.Magnetic activated carbon/macroporous resin ternary composite material(Fe_3O_(4)/AC/MR) was self-assembled using chemical co-precipitation method,and used as an adsorbent to achieve the separation and enrichment of gastrodin in urine samples.Under the optimal experimental conditions,there is a good linear relationship between the concentration of gastrodin and the peak area,with a linear range of 3.0×10^(-2)~10.0 μg·mL^(-1),linear correlation coefficient 0.9994,detection limit 5.4 μg·L^(-1).Using simulated human urine as an actual sample for spiked recovery validation,the spiked recovery rate was 94.87%~106.3%,and the relative standard deviation(RSD) was between 2.8%~4.2%.The method is convenient,fast,and effective,providing a new method for the separation and purification of gastrodin in biological samples.

关 键 词：天麻素 化学共沉淀 磁分散固相萃取 高效液相色谱法 生物样本 

分 类 号：O657.7[理学—分析化学]