miR-365a-3p通过TGF-β信号通路影响血管内皮细胞功能参与子痫前期的发病机制  

MiR⁃365a⁃3p affects vascular endothelial cell function through TGF⁃βsignaling pathway and participates in the pathogenesis of preeclampsia

作　　者：严兆华 郑健彬 张娜 曹春燕 颜露春 YAN Zhaohua;ZHENG Jianbin;ZHANG Na;CAO Chunyan;YAN Luc-hun(Department of Obstetrics and Gynecology,Affiliated Hospital of Guangdong Medical University,Zhanjiang 524001,China;不详)

机构地区：[1]广东医科大学附属医院妇产科,广东湛江524001 [2]广东医科大学附属医院检验科,广东湛江524001

出　　处：《实用医学杂志》2024年第16期2263-2269,共7页The Journal of Practical Medicine

基　　金：广东省医学科研基金项目(编号:B2019007)。

摘　　要：目的探究微小RNA(miRNA)-365a-3p影响血管内皮细胞功能参与子痫前期(PE)的发病机制。方法分离原代人脐静脉内皮细胞(HUVECs),设为NC组(转染miR-365a-3p NC)、mimics组(转染miR-365a-3p mimics)、inhibitor组(转染miR-365a-3p inhibitor),另取对数期细胞设为空白组。检测各组增殖、迁移及血管形成能力。双荧光素酶实验验证miR-365a-3p与下游基因的靶向关系。检测各组TGF-β_(1)、Smad4、Smad7蛋白表达。结果与空白组、NC组比较,mimics组24、48、72 h吸光度值及迁移率降低(P<0.05),每个视野的管状结构数量减少(P<0.05),inhibitor组24、48、72 h吸光度值及迁移率升高(P<0.05),每个视野的管状结构数量增加(P<0.05)。双荧光素酶实验表明Smad7是miR-365a-3p的一个靶基因。与空白组、NC组比较,mimics组转化生长因子-β_(1)(TGF-β_(1))、Smad4蛋白表达升高(P<0.05),Smad7蛋白表达降低(P<0.05),inhibitor组TGF-β_(1)、Smad4蛋白表达降低(P<0.05),Smad7蛋白表达升高(P<0.05)。结论miR-365a-3p可能通过调控下游TGF-β信号通路影响血管内皮细胞功能,从而参与PE发病。Objective To explore the mechanism of microRNA(miRNA)-365a-3p affecting the function of vascular endothelial cells involved in the pathogenesis of preeclampsia(PE).Methods Primary human umbilical vein endothelial cells(HUVECs)were set as a NC group(transfected miR-365a-3p NC),a mimics group(trans⁃fected miR-365a-3p mimics)and a inhibitor group(transfected miR-365a-3p inhibitor).Logarithmic HUVECs cells were set as the blank group.The cell proliferation,migration and angiogenesis in each group were detected.Dual luciferin assay verified the targeting relationship between miR-365a-3p and downstream gene.The protein expressions of TGF-β_(1),Smad4 and Smad7 in each group were detected.Results Compared with the blank group and the NC group,the absorbance value and mobility of 24,48 and 72 h were decreased(P<0.05),the number of tubular structures per field were decreased in the mimics group(P<0.05),the absorbance value and mobility of 24,48 and 72 h were increased(P<0.05),and the number of tubular structures per field were increased in the inhibitor group(P<0.05).Dual luciferin assay showed that Smad7 was a target gene of miR-365a-3p.Compared with the blank group and the NC group,the protein expressions of TGF-β_(1) and Smad4 in the mimics group were increased(P<0.05),while the protein expression of Smad7 was decreased(P<0.05).The protein expression levels of TGF-β_(1) and Smad4 in the inhibitor group were decreased(P<0.05),while the protein expression levels of Smad7 were increased(P<0.05).Conclusion miR-365a-3p may affect the function of vascular endothelial cells by regulating the downstream TGF-βsignaling pathway,and thus participate in the pathogenesis of PE.

关 键 词：子痫前期 微小RNA-365a-3p 血管内皮细胞功能 转化生长因子-Β 

分 类 号：R714.245[医药卫生—妇产科学]