真武汤加地龙联合顺铂对恶性胸腔积液模型小鼠血清白细胞介素7、血管内皮生长因子水平的影响  

Effect of Zhenwu Decoction plus geosaurus combined with cisplatin on the levels of interleukin-7 and vascular endothelial growth factor of mice with malignant pleural effusion

作　　者：王鹏 刘松江[2] 李雨[2] 闫珺[2] 张俐佳 孙姮 WANG Peng;LIU Songjiang;LI Yu;YAN Jun;ZHANG Lijia;SUN Heng(Graduate School,Heilongjiang University of Chinese Medicine,Harbin,Heilongjiang 150040;Department of Oncology,First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin,Heilongjiang 150040)

机构地区：[1]黑龙江中医药大学研究生院,黑龙江哈尔滨150040 [2]黑龙江中医药大学附属第一医院肿瘤科,黑龙江哈尔滨150040

出　　处：《河北中医》2024年第8期1315-1318,1324,共5页Hebei Journal of Traditional Chinese Medicine

基　　金：黑龙江省中医药科研项目(编号:ZHY2020-109);2019年黑龙江中医药大学科研基金项目(编号:2019MS07)。

摘　　要：目的观察真武汤加地龙联合顺铂给药对恶性胸腔积液模型小鼠血清白细胞介素7(IL-7)、血管内皮生长因子(VEGF)的影响。方法选取8周龄的雌性C57BL/6小鼠40只,适应性喂养1周后随机均分为空白对照组、模型对照组、顺铂组、真武汤加地龙组、真武汤加地龙+顺铂组,每组8只。除空白对照组外,其余4组的32只均采用LLC小鼠肺癌细胞建立小鼠恶性胸腔积液模型。建模成功3天后,顺铂组予顺铂溶液6 mg/kg腹腔注射,隔3日1次,共3次;真武汤加地龙组予33.78 g/kg的真武汤加地龙溶液灌胃,每日1次,连续10天;真武汤加地龙+顺铂组予顺铂溶液6 mg/kg腹腔注射,隔3日1次,共3次,联合33.78 g/kg的真武汤加地龙溶液灌胃,每日1次,连续10天;空白对照组和模型对照组分别予等容积0.9%氯化钠注射液灌胃,每日1次,连续10天。给药结束后,常规麻醉小鼠,暴露胸腔,用注射器抽取胸水,精确到0.1 mL,记录胸水量。采用酶联免疫吸附法检测各组小鼠血清中IL-7、VEGF含量。结果与空白对照组比较,模型对照组和各药物治疗组胸水量均升高(P<0.05);与模型对照组比较,各药物治疗组胸水量均下降(P<0.05)。真武汤加地龙+顺铂组效果最佳,优于真武汤加地龙组和顺铂组(P<0.05)。与空白对照组比较,模型对照组和各药物治疗组血清VEGF水平均升高(P<0.05);与模型对照组比较,各药物治疗组血清VEGF水平均降低(P<0.05);真武汤加地龙+顺铂组血清VEGF水平低于真武汤加地龙组和顺铂组(P<0.05)。与空白对照组比较,模型对照组和各药物治疗组血清IL-7水平均降低(P<0.05);与模型对照组比较,真武汤加地龙组和真武汤加地龙+顺铂组血清IL-7水平均升高(P<0.05),顺铂组血清IL-7水平降低(P<0.05);真武汤加地龙组和真武汤加地龙+顺铂组血清IL-7水平高于顺铂组(P<0.05);真武汤加地龙组血清IL-7水平高于真武汤加地龙+顺铂组(P<0.05)。结论真武汤加地龙联Objective To investigate the effect of combined administration of Zhenwu Decoction plus geosaurus and cisplatin on interleukin-7(IL-7)and vascular endothelial growth factor(VEGF)of mice with malignant pleural effusion(MPE).Methods A MPE model was established in C57BL/6 mice with Lewis lung carcinoma(LLC).Forty 8-week-old female C57BL/6 mice were habituated for 1 week,and they were randomly assigned into blank control group,model group,cisplatin group,Zhenwu Decoction plus geosaurus group and Zhenwu Decoction plus geosaurus+cisplatin group,with 8 mice in each group.In addition to mice in the blank control group,32 mice in the remaining groups were used to establish a MPE model using LLC cells.Mice in the blank control and model group were given 0.9%sodium chloride injections once a day for 10 days.Three days after successful modeling,mice in the cisplatin group were intraperitoneally injected with diluted cisplatin injection at 6 mg·kg-1·d-1,once every 3 days for 3 days.Mice in the Zhenwu Decoction plus geosaurus group were given 33.78 g·kg-1·d-1 crude drug solution once a day for 10 days.Mice in the Zhenwu Decoction plus geosaurus+cisplatin group were intraperitoneally injected with 6 mg·kg-1·d-1 cisplatin injection,once every 3 days,and intragastatically administrated with 33.78 g·kg-1·d-1 crude drug solution once a day for 10 days.Mice were routinely anesthetized to expose the chest,and 0.1 mL of pleural fluid was extracted with a syringe.The volume of pleural fluid was recorded.Serum levels of IL-7 and VEGF were detected by enzyme-linked immunosorbent assay(ELISA).Results Compared with the blank control group,the remaining groups had significantly increased chest water volume(P<0.05).Compared with the model control group,the drug treatment groups presented significantly decreased chest water volume(P<0.05),Zhenwu Decoction plus geosaurus+cisplatin group was superior to Zhenwu Decoction plus geosaurus group and cisplatin group for chest water volume(all P<0.05).Compared with the model blank group,the re

关 键 词：真武汤 地龙 顺铂 恶性胸腔积液 动物模型 动物实验 中西医结合 白细胞介素7 血管内皮生长因子 

分 类 号：R561.3[医药卫生—呼吸系统] R285.5[医药卫生—内科学]