Bio-VOC^(TM)呼吸采样器样品转移流程评价及优化  

Evaluation and optimization of sample transfer process for Bio-VOC^(TM) breath sampler

作　　者：张晓宇 刘喆[1,2] 董小艳[1] 王秦[1,2] ZHANG Xiaoyu;LIU Zhe;DONG Xiaoyan;WANG Qin(National Institute of Environmental Health,Chinese Center for Disease Control and Prevention,Beijing 100021,China;China CDC Key Laboratory of Environment and Population Health,Beijing 100021,China)

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所,北京100021 [2]中国疾病预防控制中心环境与人群健康重点实验室,北京100021

出　　处：《地球环境学报》2024年第3期526-534,共9页Journal of Earth Environment

基　　金：中国疾病预防控制中心环境与健康监测评价体系建设项目(23jcpj);国家自然科学基金项目(92043201)。

摘　　要：人体呼出气中挥发性有机化合物(VOCs)分析已应用于环境暴露评估和疾病诊断等领域,其中肺泡气可以排除来自周围环境以及口腔和鼻腔内死腔气体的干扰,准确反映暴露及内源性代谢物水平。对采集肺泡气的Bio-VOC^(TM)呼吸采样器转移样品至吸附管的常规操作流程进行评价及优化。使用Bio-VOC^(TM)呼吸采样器采集含有丙酮、异戊二烯、乙醇、正丁醇、正己烷和正庚烷6种VOCs的呼出气模拟样品,比较不同方式转移至吸附管对模拟样品转移效率的影响,采用热脱附-气相色谱-质谱法(TD-GC-MS)对吸附管中样品进行分析,并将优化转移方式应用于实际人群呼出气样品分析。结果表明:按照Bio-VOC^(TM)呼吸采样器常规转移方式,模拟样品中6种VOCs的转移效率均低于50%且不稳定;降低转移流量后,转移效率有所提高,进一步优化后转移效率可达90.0%—101.0%,相对标准偏差均小于5%。转移方式优化后,检测到的人体呼出气样品中3种VOCs的峰面积响应值显著提高(P<0.01)。优化方式提高了使用Bio-VOC^(TM)呼吸采样器采集肺泡气进行VOCs分析的准确性和可靠性,为肺泡气采集方法的标准化提供了科学依据。Background,aim,and scope Examination of volatile organic compounds(VOCs)in human exhaled breath finds utility in both environmental exposure assessment and disease diagnosis.Alveolar gas,a constituent of human exhalation,serves as a reflection of both exogenous exposure and endogenous metabolism,while minimizing interference from external environmental gases and dead space gas in the oral and nasal cavities.The Bio-VOC^(TM) breath sampler stands out as specialized commercial equipment designed for collecting alveolar gas.The gas sample collected by this sampler requires transfer to an adsorption tube for GC-MS detection,rendering the transfer efficiency of the sample from the sampler to the adsorption tube a crucial indicator for evaluating the sampler’s collection efficiency.Despite the lack of literature on the transfer efficiency of Bio-VOC^(TM) breath samplers,this study undertakes an evaluation of the sample transfer efficiency of the Bio-VOC^(TM) breath sampler and optimizes its traditional transfer process.Materials and methods The Bio-VOC^(TM) breath sampler was utilized for collecting simulated exhaled breath samples containing ethanol,acetone,isoprene,n-butanol,n-hexane,and n-heptane.A comparison was conducted between the transfer efficiency observed under traditional and optimized transfer processes.The samples transferred to the adsorption tubes underwent analysis using thermal desorption-gas chromatography-mass spectrometry(TD-GC-MS).Subsequently,the optimization method was implemented to analyze the exhaled breath of the population.Results During traditional operation,the transfer efficiency remains below 50%,with a relative standard deviation(RSD)ranging from 11.3%to 15.6%.However,enhancements are evident by reducing the transfer flow.Following additional optimization,transfer efficiencies reach levels between 90.0%and 101.0%,with an RSD below 5%.Statistical analysis reveals a notable increase in the peak area of VOCs collected using the optimized transfer method(P<0.01).Discussion In conventiona

关 键 词：呼出气 Bio-VOC^(TM) 吸附管 转移效率 优化 

分 类 号：R12[医药卫生—环境卫生学]