黄芩苷PLGA纳米粒的制备及其抗肝癌的体外评价  

Preparation of Baicalin-loaded PLGA Nanoparticles and Its in Vitro Evaluation for Treating Liver Cancer

作　　者：汪洁 顾雪梅 范丹丹 王丽媛 吕志阳 陈璟 WANG Jie;GU Xue-mei;FAN Dan-dan;WANG Li-yuan;LV Zhi-yang;CHEN Jing(Hanlin College of Nanjing University of Chinese Medicine,Jiangsu Taizhou 225300,China)

机构地区：[1]南京中医药大学翰林学院,江苏泰州225300

出　　处：《广州化工》2024年第13期21-24,共4页GuangZhou Chemical Industry

基　　金：2024年江苏高校青蓝工程优秀教学团队资助项目;2023年江苏省大学生创新创业项目(202313981004Y、202313981011Y);泰州市科技支撑项目(TS202232、TS202325)。

摘　　要：采用乳化溶剂挥发法制备纳米粒,选取PLGA为载体材料,PVA溶液为水相,黄芩苷为主药,以包封率为评价指标,进行单因素考察和响应面优化,筛选出黄芩苷PLGA纳米粒的最佳制备工艺,并考察其体外释放度行为。最后,通过开展细胞试验,对黄芩苷PLGA纳米粒进行体外药效学评价。结果表明,最优工艺及处方制备出的黄芩苷PLGA纳米粒,体外释放具有缓释性,对肝癌细胞具有显著抑制作用。Nanoparticles were prepared using emulsion-solvent evaporation method,with PLGA as the carrier material,PVA solution as the aqueous phase,and baicalin as the main drug.The encapsulation efficiency was used as the evaluation indicator,and a single-factor investigation and response surface optimization were conducted to screen the optimal preparation process for baicalin-loaded PLGA nanoparticles and examine their in vitro release behavior.Finally,cell experiments were conducted to evaluate the in vitro pharmacodynamics of baicalin-loaded PLGA nanoparticles.The results showed that the baicalin-loaded PLGA nanoparticles prepared using the optimal process and formula exhibited sustained release in vitro and demonstrated significant inhibitory effects on liver cancer cells.

关 键 词：黄芩苷 PLGA 纳米粒 体外释放度 肝癌 

分 类 号：TQ460.4[化学工程—制药化工]