血浆脂蛋白琼脂糖凝胶电泳综合实验设计  

Comprehensive Experiment Design of Lipoprotein Agarose Gel Electrophoresis

作　　者：冯俊荣[1] 唐永政[1] 高慧鑫 FENG Jun-rong;TANG Yong-zheng;GAO Hui-xin(College of Ocean,Yantai University,Shandong Yantai 264005,China)

机构地区：[1]烟台大学海洋学院,山东烟台264005

出　　处：《广州化工》2024年第13期162-164,共3页GuangZhou Chemical Industry

基　　金：山东省本科教学改革研究项目(No:M2021124);烟台大学教学改革项目(No:jyxm2020011)。

摘　　要：血浆脂蛋白琼脂糖凝胶电泳实验是生物化学实验课程中的经典实验,但因其操作单一、等待时间过长,学生的收获感不强,有必要进行综合设计。把上课时间分为三个时段,在各个时段设计多种学习活动:串讲原理、做练习题使相关知识融会贯通;阅读文献,提出疑问;设置试误样品组、做相关实验,启发学生顿悟。通过这些学习活动,学生不仅学会了电泳技术的基本操作,还深刻理解了实验原理,强化了勇于探索的科学精神,体会了顿悟的快乐。The experiment of plasma lipoprotein agarose gel electrophoresis was a classic experiment in biochemistryexperiment course, but because of its simple operation and long waiting time, students' sense of harvest was not strong.Therefore, it was necessary to carry out comprehensive design. The class time was divided into three periods, and avariety of learning activities were designed in each period: introducing principles and doing exercises to make relevantknowledge integrated, read the literature and ask questions, set up trial and error sample groups and do relevantexperiments to inspire students' insight and solve questions. These learning activities not only let students learn the basicoperation of electrophoresis technology, but also let students deeply understand the experimental principle, andstrengthened the scientific spirit of bold exploration, experience the joy of insight.

关 键 词：血浆脂蛋白 琼脂糖凝胶电泳 苏丹黑 综合实验设计 试误教学 

分 类 号：G642.1[文化科学—高等教育学]