Efficacy of Pudilan Xiaoyan Oral Liquid in the treatment of pneumonia induced by drug-resistant Pseudomonas aeruginosa  

作　　者：Jing Ma Yan-Yan Bao Xiao-Rui Wang Kuok Kuantai Leong-Man Chon Xiao-Lan Cui Ju-Ying Zhong Yu-Jing Shi 

机构地区：[1]Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China [2]Conde S.Januário Hospital(CHCSJ),pharmacy department,Macao 999078,China [3]Lin Fong Charities Traditional Chinese Medicine Treatment Centre,Macao 999078,China [4]Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China

出　　处：《Infectious Diseases Research》2024年第3期22-29,共8页传染病研究（英文）

基　　金：supported by Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences(No.CI2021B015);the Fundamental Research Funds for the Central Public Welfare Research Institutes(JJPY2022017).

摘　　要：Background:Pudilan Xiaoyan Oral Liquid(PDL)is a Chinese patent medicine with notable pharmacological properties,including anti-inflammatory and antibacterial effects.Drug-resistant Pseudomonas aeruginosa infection is a common and refractory bacterial infection in clinical practice.Due to its high drug resistance,it brings great challenges to treatment.This study aimed to assess the therapeutic efficacy of PDL in a murine model of pneumonia induced by drug-resistant Pseudomonas aeruginosa.Methods:Three different doses of PDL(11 mL/kg/d,5.5 mL/kg/d,2.75 mL/kg/d)were used to observe lung tissue pathology and inflammatory cytokine levels in pneumonia mouse models induced by multidrug-resistant Pseudomonas aeruginosa(MDR-PA).Additionally,the protective efficacy of PDL against mortality in infected mice was evaluated using a death model caused by MDR-PA.Finally sub-MIC concentration of levofloxacin was used to induce drug-resistant mice pneumonia model to evaluate the role of PDL in reversing drug resistance.Experimental data are expressed as mean±standard deviation.Statistical significance was determined by one-way analysis of variance followed by Tukey’s multiple-comparisons test.Results:Treatment effect of PDL on MDR-PA pneumonia:the medium and small doses of PDL can significantly reduce the lung index of multi-drug resistant bacteria infected pneumonia model mice(P<0.05),the lung index inhibition rates for these groups were 55.09%and 58.43%,and improve the degree of lung tissue lesions of mice;The expression of serum cytokines keratinocyte chemoattractant,tumor necrosis factor-αand monocyte chemoattractant protein-1 could be decreased in the three dosage groups of PDL(P<0.01).PDL treatment not only lowered the mortality but also extended the survival duration in mice infected with MDR-PA.It was found after sub-MIC concentration of levofloxacin induced resistance of Pseudomonas aeruginosa to pneumonia in mice.Compared with the model group,the lung index of mice in high and medium PDL doses was significantly redu

关 键 词：PUDILAN Pseudomonas aeruginosa drug resistance mice model 

分 类 号：R285[医药卫生—中药学]