Isolation and Experimental Study on Bacteriostasis of Bacillus haynesii  

作　　者：Jiedan Liao Mengbo Yu Jiaming Li Zhiheng Xie Wanjun Gu Shuzhen Zhou Yi Ma 

机构地区：[1]College of Animal Science and Technology,Foshan University,Foshan 528225,Guangdong Province,China [2]Foshan City Nanhai Eastern Along Pharmaceutical Co.,Ltd,Foshan 528234,Guangdong Province,China [3]Department of Veterinary Medicine,College of Coastal Agricultural Sciences,Guangdong Ocean University,Zhanjiang 524088,Guangdong Province,China

出　　处：《Journal of Clinical and Nursing Research》2024年第7期219-227,共9页临床护理研究（英文）

基　　金：Natural Science Foundation of Guangdong Province in 2023(No.2023A1515012181);Self-funded Science and Technology Innovation Project of Foshan City in 2022(No.220001005797);Basic and Applied Basic Research Foundation of Guangdong Province in 2022(No.2022A1515140052);Innovation Project of Guangdong Graduate Education in 2022(No.2022JGXM129,No.2022JGXM128)and 2023(No.2023ANLK-080)。

摘　　要：This experiment aims to isolate and inhibit three bacteria strains to provide candidate strains for the development and application of probiotics.Using bacterial morphological identification,16S rDNA sequence alignment,and genetic evolution analysis,three strains were identified as Bacillus haynesii,named HP01,HD02,and HK03.Through biosurfactant activity tests,C-TAB tests,hemolysis tests,and antibacterial activity analyses,the results showed that all three strains of B.haynesii exhibited significant biosurfactant activity.Additionally,the solutions of the three strains demonstrated a pronounced antibacterial effect on Staphylococcus aureus.The resistance and safety of commonly used drugs were evaluated using the tablet diffusion method and a mouse feeding test.The results indicated that the three strains were not resistant to commonly used antibacterial drugs,and the oral bacterial solution was not pathogenic and had high safety in mice.The study concluded that all three B.haynesii strains met the basic conditions for use,with B.haynesii HP01 being the most promising candidate.

关 键 词：Bacillus haynesii Separation identification Antibacterial activity Safety 

分 类 号：Q93[生物学—微生物学]