石墨消解仪-分光光度法测定金顶侧耳中的总糖含量  

Determination of Total Sugar Content in Pleurotus citrinopileatus by Graphite Digestion and Spectrophotometry

作　　者：曹燕妮 华蓉 刘韵然[1] 游金坤 王娟[2] 杨璐敏 孙达锋 CAO Yanni;HUA Rong;LIU Yunran;YOU Jinkun;WANG Juan;YANG Lumin;SUN Dafeng(Yunnan Academy of Edible Fungi Industry Development,Kunming 650221,China;Kunming Edible Fungi Institute of All China Federation of Supply and Marketing Cooperatives,Kunming 650221,China)

机构地区：[1]云南省食用菌产业发展研究院,云南昆明650221 [2]中华全国供销合作总社昆明食用菌研究所,云南昆明650221

出　　处：《中国食用菌》2024年第4期70-73,共4页Edible Fungi of China

基　　金：科技人才与平台计划(院士专家工作站)(202305AF150187);科技人才与平台计划(202405AD350043)。

摘　　要：试验以盐酸浓度、盐酸添加量、水解温度、水解时间优化石墨消解仪水解条件,测定了金顶侧耳中的总糖得率。结果表明,盐酸浓度为3 mol·L^(-1)、盐酸体积为40 mL、水解温度为140℃、水解时间为120 min时,金顶侧耳中总糖得率最高,方法学验证其具有良好的精密度和准确度。对有证标准物质的实测值在参考范围内,验证了此方法的可行性。The Pleurotus citrinopileatus of total sugar yield were determinated by graphite digestion,and the hydrolysis conditions of graphite digestion were optimized by hydrochloric acid concentration,hydrochloric acid addition,hydrolysis temperature and hydrolysis time.The results showed that the highest total sugar yield was obtained when the hydrochloric acid concentration was 3 mol·L^(-1),the volume of hydrochloric acid was 40 mL,the hydrolysis temperature was 140°C,and the hydrolysis time was 120 min.Methodological validation showed good precision and accuracy.The measured value was within the reference range for the certified reference materials,which verified the feasibility of this method.

关 键 词：金顶侧耳 总糖 石墨消解 水解 

分 类 号：S646.1[农业科学—蔬菜学]