白色念珠菌水溶物诱导的川崎病模型心脏与脾脏病理学特征  

Pathological characteristics of heart and spleen in a kawasaki disease model induced by candida albicans watersoluble fraction

作　　者：张嘉鑫 张宗丽 罗一帆 李涛[1] 席世兵[1] ZHANG Jiaxin;ZHANG Zongli;LUO Yifan;LI Tao;XI Shibing(Department of Pediatrics,2Pediatric Disease Research Institute,Taihe Hospital,Hubei University of Medicine,Shiyan 442000,China)

机构地区：[1]湖北医药学院附属太和医院儿科,十堰442000 [2]湖北医药学院附属太和医院儿科疾病研究所,十堰442000

出　　处：《临床与实验病理学杂志》2024年第7期719-724,共6页Chinese Journal of Clinical and Experimental Pathology

基　　金：湖北省儿科科研基金(湖北省儿科联盟科研项目)(HPAMRP202107)。

摘　　要：目的 探讨白色念珠菌水溶物(candida albicans watersoluble fraction, CAWS)诱导的川崎病(Kawasaki disease, KD)模型心脏与脾脏病理学特征,为KD病理机制提供研究基础。方法 采用腹腔注射CAWS构建C57BL/6小鼠KD模型,取心脏、主动脉和脾脏标本,测体、心脏和脾脏质量;应用HE染色观察心脏、主动脉和脾脏的形态学变化;运用qRT-PCR和免疫荧光法检测脾脏中成熟巨噬细胞标志物(F4/80)、TNF-α和IL-1β的表达水平,分析其临床病理学特征并复习相关文献。结果 与对照组相比,模型组小鼠的心脏和体质量无明显差异,但脾脏质量明显增加(P<0.05)。模型组小鼠的心肌间质出现单核细胞浸润,主动脉血管壁的弹性纤维结构紊乱并伴黏液样变性,脾脏红白髓结构紊乱,大量巨噬细胞浸润。模型组小鼠脾脏F4/80(2.58倍,P<0.000 1)、TNF-α(1.43倍,P<0.001)、IL-1β(3.62倍,P<0.000 1)的mRNA水平和免疫荧光染色强度均显著高于对照组。结论 CAWS诱导的KD模型小鼠心脏和主动脉出现类似KD的病理变化,脾脏显著肿大、巨噬细胞浸润并伴TNF-α和IL-1β表达增加。Purpose To investigating the pathological characteristics of the heart and spleen in a Kawasaki disease(KD)model induced by candida albicans watersoluble fraction(CAWS)and to provide a research basis for the pathological mechanisms of KD.Methods Intraperitoneal injection of CAWS was used to establish a KD model in C57BL/6 mice.Specimens of the heart,aorta,and spleen were collected to measure body weight and the weights of the heart and spleen.HE staining was utilized to examine the morphological alterations in the heart,aorta,and spleen.The expression levels of the mature macrophage marker(F4/80),TNF-α,and IL-1βin the spleen were measured using qRT-PCR and immunofluorescence.its clinical pathological characteristics were analyze and relevant literatures were reviewed.Results Compared to the control group,there were no significant differences in the heart and body mass of the model group mice,but their spleen mass significantly increased(P<0.05).In the myocardial interstitium of model group mice,there was infiltration of mononuclear cells,disorder of the elastic fibers in the aortic wall along with mucoid degeneration,disruption of the splenic red and white pulp structure,and significant macrophage infiltration.In the model group mice,the mRNA levels and immunofluorescence staining intensity of spleen F4/80(2.58-fold,P<0.0001),TNF-α(1.43-fold,P<0.001),and IL-1β(3.62-fold,P<0.0001)were significantly higher than those in the control group.Conclusion Mice with KD models induced by CAWS can exhibit pathological alterations akin to KD in the heart and aorta,significant enlargement of the spleen,infiltration by macrophages,with elevated expressions of TNF-αand IL-1β.

关 键 词：川崎病 白色念珠菌水溶物 脾肿大 巨噬细胞浸润 

分 类 号：R725.4[医药卫生—儿科]