正交试验优化鸡矢藤总环烯醚萜苷的提取工艺  

Optimization of Extraction Technology of Total Iridoid Glycosides from Paederia scandens(Lour.)Merr.by Orthogonal Experiment

作　　者：陈冉[1] 雷雨彩 赵柳双 黄锁义[1] 孙科[1] CHEN Ran;LEI Yu-cai;ZHAO Liu-shuang;HUANG Suo-yi;SUN Ke(Youjiang Medical University for Nationalities,Guangxi Baise 533000;Chongzuo Meteorological Bureau of Guangxi Zhuang Autonomous Region,Guangxi Chongzuo 532200,China)

机构地区：[1]右江民族医学院,广西百色533000 [2]广西壮族自治区崇左市气象局,广西崇左532200

出　　处：《广州化工》2024年第14期49-52,共4页GuangZhou Chemical Industry

基　　金：广西高校中青年教师科研基础能力提升资助项目(2019KY0554,2019KY0572);广西重点研发计划资助项目(桂科AB18221095)。

摘　　要：为研究鸡矢藤叶中总环烯醚萜苷的最佳提取工艺,以总环烯醚萜苷提取率为指标,通过单因素试验和正交试验,考察乙醇浓度、提取时间、料液比、提取温度、提取次数等因素的影响。结果显示总环烯醚萜苷的最佳提取条件为:80%乙醇作为提取溶剂、提取时间2 min、料液比1∶90 g/mL、提取温度45℃、提取次数6次,在此条件下总环烯醚萜苷的提取率达到46.59 mg/g。本文研究的提取方法高效、稳定、合理,可为鸡矢藤叶中总环烯醚萜苷的研究奠定一定基础。To optimize the extraction technology of total iridoid glycosides from Paederia scandens(Lour.)Merr.leaf,the extraction rate of total iridoid glycosides as an indicator was determined.The effect of the extraction temperature,extraction time,solid-liquid ratio,extraction temperature,extraction times were studied by using single factor experiment and orthogonal experiment.The results indicated that the optimal extraction conditions for total iridoid glycosides were 80%ethanol concentrationas extraction solvent,extraction time of 2 min,solid-liquid ratio of 1∶90 g/mL,extraction temperature of 45℃and extraction times of 6 times.Under the optimum conditions,the extraction rate of total iridoid glycosides could reach 46.59 mg/g.The selected extraction process is efficient,stable,and reasonable,which can lay an experimental foundation for the study of total iridoid glycosides from Paederia(Lour.)Merr.Leaf.

关 键 词：鸡矢藤 总环烯醚萜苷 正交实验 提取工艺 

分 类 号：R284.2[医药卫生—中药学]