TLR3基因单核苷酸多态性与儿童紫癜性肾炎的相关性  

Association of single nucleotide polymorphisms of the TLR3 gene with Henoch-Sch nlein purpura in children

作　　者：屈凤祥 常红[1] 林毅[1] QU Fengxiang;CHANG Hong;LIN Yi(Department of Pediatrics,The Affiliated Hospital of Qingdao University,Qingdao 266003,China)

机构地区：[1]青岛大学附属医院儿科,山东青岛266003

出　　处：《精准医学杂志》2024年第4期352-355,共4页Journal of Precision Medicine

基　　金：青岛大学附属医院临床医学+X科研项目(2019+X017)。

摘　　要：目的探讨TLR3基因单核苷酸多态性与儿童过敏性紫癜(Henoch-Schönlein purpura,HSP)及紫癜性肾炎(Henoch-Sch nleinöpurpura nephritis,HSPN)易感性的相关性。方法选择HSP患儿174例作为病例组,选择同期体检的162例健康儿童作为对照组。根据病例组患儿在随访过程中是否合并肾脏损害分为HSP组、HSPN组。采用多重聚合酶链反应技术(M-PCR)靶向捕获TLR3基因rs35311343、rs121434431、rs199768900、rs768091235、rs1244010954位点,通过高通量测序技术对所有样本的上述位点进行测序,根据测序结果进行各位点基因型以及基因频率的统计分析。结果病例组与对照组TLR3基因rs35311343、rs121434431、rs199768900、rs768091235、rs1244010954位点的各基因型频率和各等位基因频率比较,差异无显著意义(P>0.05)。病例组中HSP组与HSPN组TLR3基因rs121434431、rs199768900、rs768091235、rs1244010954位点的各基因型频率和各等位基因频率比较,差异无显著性(P>0.05);rs35311343位点的基因型频率与等位基因频率比较差异有显著性(χ^(2)=9.492,OR=2.662,95%CI=1.342~5.281,P<0.05)。结论TLR3基因rs35311343位点CG基因型与儿童过敏性紫癜肾脏受累有关,等位基因G可能是HSPN的易感基因。Objective To investigate the association of single nucleotide polymorphisms of the TLR3 gene with susceptibility to Henoch-Schönlein purpura(HSP)and Henoch-Schönlein purpura nephritis(HSPN)in children.Methods A total of 174 children with HSP were enrolled as case group,and 162 healthy children who underwent physical examination during the same period of time were enrolled as control group.The children in the case group were divided into HSP group and HSPN group accor-ding to the presence or absence of renal damage during follow-up.Multiplex polymerase chain reaction was used for targeted capture of the rs35311343,rs121434431,rs199768900,rs768091235,and rs1244010954 loci of the TLR3 gene,high-throughput sequencing was performed for the above loci of all samples,and a statistical analysis was performed for the genotype and gene frequency of each locus.Results There were no significant differences in the genotype and allele frequencies of the rs35311343,rs121434431,rs199768900,rs768091235,and rs1244010954 loci of the TLR3 gene between the case group and the control group(P>0.05).For the case group,there were no significant differences in the genotype and allele frequencies of the rs121434431,rs199768900,rs768091235,and rs1244010954 loci of the TLR3 gene between the HSP group and the HSPN group(P>0.05),while there was a significant difference in the genotype and allele frequencies of the rs35311343 locus between the two groups(χ^(2)=9.492,OR=2.662,95%CI=1.342-5.281,P<0.05).Conclusion The CG genotype of the rs35311343 locus of the TLR3 gene is associated with renal involvement in children with HSP,and allele G may be a susceptibility gene for HSPN.

关 键 词：紫癜 过敏性 TOLL样受体3 多态性 单核苷酸 肾炎 高通量核苷酸序列分析 儿童 

分 类 号：R726.92[医药卫生—儿科] R554.6[医药卫生—临床医学]