活血补肾安神方对氧化应激损伤H9c2心肌细胞能量代谢的影响  

Effects of Huoxue Bushen Anshen Prescription on Energy Metabolism of H9c2 Cardiomyocytes with Oxidative Stress Injury

作　　者：程诺 王阶[2] 刘兰椿 程嘉雯 CHENG Nuo;WANG Jie;LIU Lanchun;CHENG Jiawen(Beijing University of Chinese Medicine,Beijing 100029,China;Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)

机构地区：[1]北京中医药大学,北京100029 [2]中国中医科学院广安门医院,北京100053

出　　处：《中国中医药信息杂志》2024年第9期94-101,共8页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(82230124)。

摘　　要：目的 观察活血补肾安神方对氧化应激损伤H9c2心肌细胞能量代谢的影响。方法 采用H_(2)O_(2)诱导H9c2细胞氧化应激模拟心肌损伤,CCK-8法筛选最佳造模条件及药物浓度。将细胞分为正常组、模型组、盐酸曲美他嗪组和活血补肾安神方组,对处理后的H9c2心肌细胞进行Na^(+)-K^(+)-ATP酶活性检测及实时ATP生成速率、线粒体压力、糖酵解速率和长链脂肪酸氧化压力测定,观察心肌细胞能量代谢变化。结果 与正常组比较,模型组H9c2细胞Na^(+)-K^(+)-ATP酶活性、糖酵解ATP生成速率及线粒体ATP生成速率、基础氧耗、非线粒体氧耗、ATP生成能力、细胞储备呼吸能力和最大呼吸能力降低,糖酵解速率减小,长链脂肪酸基础呼吸、急性响应及最大呼吸值降低(P<0.05,P<0.01);与模型组比较,活血补肾安神方组H9c2细胞Na^(+)-K^(+)-ATP酶活性升高,糖酵解ATP生成速率及线粒体ATP生成速率增大,基础氧耗、非线粒体氧耗、ATP生成能力、细胞储备呼吸能力及最大呼吸能力升高,基础糖酵解速率增大,长链脂肪酸基础呼吸、急性响应及最大呼吸值升高(P<0.05,P<0.01)。结论 活血补肾安神方可显著改善氧化应激损伤H9c2心肌细胞Na^(+)-K^(+)-ATP酶活性,提高ATP生成速率,改善线粒体压力、糖酵解速率及长链脂肪酸氧化压力,发挥改善心肌细胞能量代谢作用。Objective To observe the effects of Huoxue Bushen Anshen Prescription on energy metabolism of H9c2 cardiomyocytes with oxidative stress injury.Methods H9c2 cardiomyocytes oxidative stress model was established with H2O2 to simulate myocardial injury.CCK-8 method was used to select the best conditions for modeling and the appropriate concentration of drugs.Cells were divided into normal group,model group,trimetazidine hydrochloride group and Huoxue Bushen Anshen Prescription group.Na^(+)-K^(+)-ATPase activity,real-time ATP generation rate,mitochondrial pressure,gycolysis rate,and long-chain fatty acid oxidationl pressure on H9c2 cardiomyocytes after treatment were performed,and the changes in energy metabolism of cardiomyocytes were observed.Results Compared with the normal group,the Na^(+)-K^(+)-ATPase activity,glycolytic ATP production rate,mitochondrial ATP production rate,basal oxygen consumption,non mitochondrial oxygen consumption,ATP production capacity,cellular reserve respiratory capacity and maximum respiratory capacity of H9c2 cells in the model group decreased,the glycolytic rate slowed down,long chain fatty acid basal respiration,acute response and maximum respiratory value decreased(P<0.05,P<0.01).Compared with the model group,the H9c2 cells in Huoxue Bushen Anshen Prescription group showed an increase in Na^(+)-K^(+)-ATPase activity,an increase in glycolytic ATP production rate and mitochondrial ATP production rate,an increase in basal oxygen consumption,non mitochondrial oxygen consumption,ATP production capacity,cellular reserve respiratory capacity and maximum respiratory capacity,an increase in basal glycolysis rate,and an increase in long-chain fatty acid basal respiration,acute response and maximum respiratory value(P<0.05,P<0.01).Conclusion Huoxue Bushen Anshen Prescription can significantly improve the activity of Na^(+)-K^(+)-ATPase in cH9c2 ardiomyocytes with oxidative stress injury,increase the rate of ATP production,improve mitochondrial pressure,glycolysis rate and oxidation pressure of

关 键 词：活血补肾安神方 H9C2心肌细胞 能量代谢 线粒体 

分 类 号：R285.5[医药卫生—中药学]